{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mola MG"],"funding":["University of Bari-Progetto di Ricerca IDEA Giovani Ricercatori","NIDDK NIH HHS","NINDS NIH HHS","Italian Ministry of the University and Research-FIRB Futuro in Ricerca"],"pagination":["139-54"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4905710"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["64(1)"],"pubmed_abstract":["Regulatory volume decrease (RVD) is a process by which cells restore their original volume in response to swelling. In this study, we have focused on the role played by two different Aquaporins (AQPs), Aquaporin-4 (AQP4), and Aquaporin-1 (AQP1), in triggering RVD and in mediating calcium signaling in astrocytes under hypotonic stimulus. Using biophysical techniques to measure water flux through the plasma membrane of wild-type (WT) and AQP4 knockout (KO) astrocytes and of an astrocyte cell line (DI TNC1) transfected with AQP4 or AQP1, we here show that AQP-mediated fast swelling kinetics play a key role in triggering and accelerating RVD. Using calcium imaging, we show that AQP-mediated fast swelling kinetics also significantly increases the amplitude of calcium transients inhibited by Gad"],"journal":["Glia"],"pubmed_title":["The speed of swelling kinetics modulates cell volume regulation and calcium signaling in astrocytes: A different point of view on the role of aquaporins."],"pmcid":["PMC4905710"],"funding_grant_id":["R01 NS092466","GRBA085SIS","P30 DK041296","RBFR12SJA8."],"pubmed_authors":["Spray DC","Svelto M","Frigeri A","Gargano CD","Scemes E","Sparaneo A","Mola MG","Nicchia GP"],"additional_accession":[]},"is_claimable":false,"name":"The speed of swelling kinetics modulates cell volume regulation and calcium signaling in astrocytes: A different point of view on the role of aquaporins.","description":"Regulatory volume decrease (RVD) is a process by which cells restore their original volume in response to swelling. In this study, we have focused on the role played by two different Aquaporins (AQPs), Aquaporin-4 (AQP4), and Aquaporin-1 (AQP1), in triggering RVD and in mediating calcium signaling in astrocytes under hypotonic stimulus. Using biophysical techniques to measure water flux through the plasma membrane of wild-type (WT) and AQP4 knockout (KO) astrocytes and of an astrocyte cell line (DI TNC1) transfected with AQP4 or AQP1, we here show that AQP-mediated fast swelling kinetics play a key role in triggering and accelerating RVD. Using calcium imaging, we show that AQP-mediated fast swelling kinetics also significantly increases the amplitude of calcium transients inhibited by Gad","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2025-04-19T23:17:22.565Z","creation":"2019-03-27T02:15:55Z"},"accession":"S-EPMC4905710","cross_references":{"pubmed":["26413835"],"doi":["10.1002/glia.22921"]}}