biostudies-literatureMola MGUniversity of Bari-Progetto di Ricerca IDEA Giovani RicercatoriNIDDK NIH HHSNINDS NIH HHSItalian Ministry of the University and Research-FIRB Futuro in Ricerca139-54https://www.ebi.ac.uk/biostudies/studies/S-EPMC4905710biostudies-literatureUnknown64(1)Regulatory volume decrease (RVD) is a process by which cells restore their original volume in response to swelling. In this study, we have focused on the role played by two different Aquaporins (AQPs), Aquaporin-4 (AQP4), and Aquaporin-1 (AQP1), in triggering RVD and in mediating calcium signaling in astrocytes under hypotonic stimulus. Using biophysical techniques to measure water flux through the plasma membrane of wild-type (WT) and AQP4 knockout (KO) astrocytes and of an astrocyte cell line (DI TNC1) transfected with AQP4 or AQP1, we here show that AQP-mediated fast swelling kinetics play a key role in triggering and accelerating RVD. Using calcium imaging, we show that AQP-mediated fast swelling kinetics also significantly increases the amplitude of calcium transients inhibited by GadGliaThe speed of swelling kinetics modulates cell volume regulation and calcium signaling in astrocytes: A different point of view on the role of aquaporins.PMC4905710R01 NS092466GRBA085SISP30 DK041296RBFR12SJA8.Spray DCSvelto MFrigeri AGargano CDScemes ESparaneo AMola MGNicchia GPfalseThe speed of swelling kinetics modulates cell volume regulation and calcium signaling in astrocytes: A different point of view on the role of aquaporins.Regulatory volume decrease (RVD) is a process by which cells restore their original volume in response to swelling. In this study, we have focused on the role played by two different Aquaporins (AQPs), Aquaporin-4 (AQP4), and Aquaporin-1 (AQP1), in triggering RVD and in mediating calcium signaling in astrocytes under hypotonic stimulus. Using biophysical techniques to measure water flux through the plasma membrane of wild-type (WT) and AQP4 knockout (KO) astrocytes and of an astrocyte cell line (DI TNC1) transfected with AQP4 or AQP1, we here show that AQP-mediated fast swelling kinetics play a key role in triggering and accelerating RVD. Using calcium imaging, we show that AQP-mediated fast swelling kinetics also significantly increases the amplitude of calcium transients inhibited by Gad2016-01-01T00:00:00Z2016 Jan2025-04-19T23:17:22.565Z2019-03-27T02:15:55ZS-EPMC49057102641383510.1002/glia.22921