{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yamada H"],"funding":["Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)","Princess Takamatsu Cancer Research Fund","Magnetic Health Science Foundation","Daiwa Securities Health Foundation","SEI Group CSR Foundation","Innovative Techno-Hub for Integrated Medical Bio-Imaging of the Project for Developing Innovation Systems","JSPS"],"pagination":["125-8"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4906467"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(2)"],"pubmed_abstract":["In an attempt to monitor μm-level trace constituents, we applied here (1)H-{(13)C-(15)N} triple-resonance nuclear magnetic resonance (NMR) to (13)C/(15)N-enriched l-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μm-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered l-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7-76 μm, which covers the typical stimulated level of ∼30 μm) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7 T MR machine for small animals. "],"journal":["ChemistryOpen"],"pubmed_title":["(13)C/(15)N-Enriched l-Dopa as a Triple-Resonance NMR Probe to Monitor Neurotransmitter Dopamine in the Brain and Liver Extracts of Mice."],"pmcid":["PMC4906467"],"funding_grant_id":["15K01819","25350977","15H01403"],"pubmed_authors":["Kimura Y","Kondo T","Matsuda T","Toshimitsu A","Sando S","Aoyama Y","Yamada H","Imai H","Kameda T"],"additional_accession":[]},"is_claimable":false,"name":"(13)C/(15)N-Enriched l-Dopa as a Triple-Resonance NMR Probe to Monitor Neurotransmitter Dopamine in the Brain and Liver Extracts of Mice.","description":"In an attempt to monitor μm-level trace constituents, we applied here (1)H-{(13)C-(15)N} triple-resonance nuclear magnetic resonance (NMR) to (13)C/(15)N-enriched l-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μm-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered l-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7-76 μm, which covers the typical stimulated level of ∼30 μm) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7 T MR machine for small animals. ","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Apr","modification":"2025-04-19T23:19:01.218Z","creation":"2019-03-27T02:15:58Z"},"accession":"S-EPMC4906467","cross_references":{"pubmed":["27308224"],"doi":["10.1002/open.201500196"]}}