biostudies-literatureYamada HMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Princess Takamatsu Cancer Research FundMagnetic Health Science FoundationDaiwa Securities Health FoundationSEI Group CSR FoundationInnovative Techno-Hub for Integrated Medical Bio-Imaging of the Project for Developing Innovation SystemsJSPS125-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC4906467biostudies-literatureUnknown5(2)In an attempt to monitor μm-level trace constituents, we applied here (1)H-{(13)C-(15)N} triple-resonance nuclear magnetic resonance (NMR) to (13)C/(15)N-enriched l-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μm-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered l-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7-76 μm, which covers the typical stimulated level of ∼30 μm) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7 T MR machine for small animals. ChemistryOpen(13)C/(15)N-Enriched l-Dopa as a Triple-Resonance NMR Probe to Monitor Neurotransmitter Dopamine in the Brain and Liver Extracts of Mice.PMC490646715K018192535097715H01403Kimura YKondo TMatsuda TToshimitsu ASando SAoyama YYamada HImai HKameda Tfalse(13)C/(15)N-Enriched l-Dopa as a Triple-Resonance NMR Probe to Monitor Neurotransmitter Dopamine in the Brain and Liver Extracts of Mice.In an attempt to monitor μm-level trace constituents, we applied here (1)H-{(13)C-(15)N} triple-resonance nuclear magnetic resonance (NMR) to (13)C/(15)N-enriched l-Dopa as the inevitable precursor of the neurotransmitter dopamine in the brain. The perfect selectivity (to render endogenous components silent) and μm-level sensitivity (700 MHz spectrometer equipped with a cryogenic probe) of triple-resonance allowed the unambiguous and quantitative metabolic and pharmacokinetic analyses of administered l-Dopa/dopamine in the brain and liver of mice. The level of dopamine generated in the brain (within the range 7-76 μm, which covers the typical stimulated level of ∼30 μm) could be clearly monitored ex vivo, but was slightly short of the detection limit of a 7 T MR machine for small animals. 2016-01-01T00:00:00Z2016 Apr2025-04-19T23:19:01.218Z2019-03-27T02:15:58ZS-EPMC49064672730822410.1002/open.201500196