biostudies-literatureHerrera VLNCATS NIH HHSNational Cancer InstituteBU CTSI awardNCI NIH HHSBU Office of Technology Development15https://www.ebi.ac.uk/biostudies/studies/S-EPMC4906906biostudies-literatureUnknown17(1)<h4>Background</h4>In contrast to rat and mouse databases, the NCBI gene database lists the human dual-endothelin1/VEGFsp receptor (DEspR, formerly Dear) as a unitary transcribed pseudogene due to a stop [TGA]-codon at codon#14 in automated DNA and RNA sequences. However, re-analysis is needed given prior single gene studies detected a tryptophan [TGG]-codon#14 by manual Sanger sequencing, demonstrated DEspR translatability and functionality, and since the demonstration of actual non-translatability through expression studies, the standard-of-excellence for pseudogene designation, has not been performed. Re-analysis must meet UNIPROT criteria for demonstration of a protein's existence at the highest (protein) level, which a priori, would override DNA- or RNA-based deductions.<h4>Methods</hBMC molecular biologyConfirmation of translatability and functionality certifies the dual endothelin1/VEGFsp receptor (DEspR) protein.PMC49069065P30CA045508P30 CA045508U54 TR001012NIH-U54TR001012Steffen MRuiz-Opazo NPappin DJTan GAPasion KAHerrera VLMoran AMRivera KfalseConfirmation of translatability and functionality certifies the dual endothelin1/VEGFsp receptor (DEspR) protein.<h4>Background</h4>In contrast to rat and mouse databases, the NCBI gene database lists the human dual-endothelin1/VEGFsp receptor (DEspR, formerly Dear) as a unitary transcribed pseudogene due to a stop [TGA]-codon at codon#14 in automated DNA and RNA sequences. However, re-analysis is needed given prior single gene studies detected a tryptophan [TGG]-codon#14 by manual Sanger sequencing, demonstrated DEspR translatability and functionality, and since the demonstration of actual non-translatability through expression studies, the standard-of-excellence for pseudogene designation, has not been performed. Re-analysis must meet UNIPROT criteria for demonstration of a protein's existence at the highest (protein) level, which a priori, would override DNA- or RNA-based deductions.<h4>Methods</h2016-01-01T00:00:00Z2016 Jun2026-06-15T07:05:34.119Z2019-03-27T02:15:59ZS-EPMC49069062730137710.1186/s12867-016-0066-8