biostudies-literatureWang XNCI NIH HHSNIGMS NIH HHS28260https://www.ebi.ac.uk/biostudies/studies/S-EPMC4908586biostudies-literatureUnknown6Although the translational function of tRNA has long been established, extra translational functions of tRNA are still being discovered. We previously developed a computational method to systematically predict new tRNA-protein complexes and experimentally validated six candidate proteins, including the mitogen-activated protein kinase kinase 2 (MEK2), that interact with tRNA in HEK293T cells. However, consequences of the interaction between tRNA and these proteins remain to be elucidated. Here we tested the consequence of the interaction between tRNA and MEK2 in pancreatic cancer cell lines. We also generated disease and drug resistance-derived MEK2 mutants (Q60P, P128Q, S154F, E207K) to evaluate the function of the tRNA-MEK2 interaction. Our results demonstrate that tRNA interacts with thScientific reportsInteraction of tRNA with MEK2 in pancreatic cancer cells.PMC4908586R01 CA186885P30 CA060553DP1 GM105386Munshi HGRosner MRPan TLee JEbine KWang XChow CRfalseInteraction of tRNA with MEK2 in pancreatic cancer cells.Although the translational function of tRNA has long been established, extra translational functions of tRNA are still being discovered. We previously developed a computational method to systematically predict new tRNA-protein complexes and experimentally validated six candidate proteins, including the mitogen-activated protein kinase kinase 2 (MEK2), that interact with tRNA in HEK293T cells. However, consequences of the interaction between tRNA and these proteins remain to be elucidated. Here we tested the consequence of the interaction between tRNA and MEK2 in pancreatic cancer cell lines. We also generated disease and drug resistance-derived MEK2 mutants (Q60P, P128Q, S154F, E207K) to evaluate the function of the tRNA-MEK2 interaction. Our results demonstrate that tRNA interacts with th2016-01-01T00:00:00Z2016 Jun2026-05-30T14:03:31.009Z2019-03-27T02:16:05ZS-EPMC49085862730142610.1038/srep28260