{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kirkconnell KS"],"funding":["National Institute of Environmental Health Sciences","NIEHS NIH HHS","NHGRI NIH HHS","NCI NIH HHS","National Institutes of Health","National Human Genome Research Institute","NIGMS NIH HHS"],"pagination":["837-47"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4920201"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(6)"],"pubmed_abstract":["Dynamic regulation of gene expression via signal transduction pathways is of fundamental importance during many biological processes such as cell state transitioning, cell cycle progression and stress responses. In this study we used serum stimulation as a cell response paradigm to apply the nascent RNA Bru-seq technique in order to capture early dynamic changes in the nascent transcriptome. Our data provides an unprecedented view of the dynamics of genome-wide transcription during the first two hours of serum stimulation in human fibroblasts. While some genes showed sustained induction or repression, other genes showed transient or delayed responses. Surprisingly, the dynamic patterns of induction and suppression of response genes showed a high degree of similarity, suggesting that these "],"journal":["Biology open"],"pubmed_title":["Capturing the dynamic nascent transcriptome during acute cellular responses: The serum response."],"pmcid":["PMC4920201"],"funding_grant_id":["T32ES007062","P30 ES017885","R01 HG006786","1R21ES020946","1R01HG006786","P50CA130810","T32GM07544","T32 GM007544","P50 CA130810","T32 ES007062","R21 ES020946"],"pubmed_authors":["Magnuson B","Paulsen MT","Ljungman M","Bedi K","Kirkconnell KS"],"additional_accession":[]},"is_claimable":false,"name":"Capturing the dynamic nascent transcriptome during acute cellular responses: The serum response.","description":"Dynamic regulation of gene expression via signal transduction pathways is of fundamental importance during many biological processes such as cell state transitioning, cell cycle progression and stress responses. In this study we used serum stimulation as a cell response paradigm to apply the nascent RNA Bru-seq technique in order to capture early dynamic changes in the nascent transcriptome. Our data provides an unprecedented view of the dynamics of genome-wide transcription during the first two hours of serum stimulation in human fibroblasts. While some genes showed sustained induction or repression, other genes showed transient or delayed responses. Surprisingly, the dynamic patterns of induction and suppression of response genes showed a high degree of similarity, suggesting that these ","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jun","modification":"2025-04-04T19:11:13.653Z","creation":"2019-06-06T15:59:23Z"},"accession":"S-EPMC4920201","cross_references":{"pubmed":["27230646"],"doi":["10.1242/bio.019323"]}}