{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hayes S"],"funding":["Natural Sciences and Engineering Research Council of Canada"],"pagination":["E172"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4926192"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(6)"],"pubmed_abstract":["The bacteriophage lambda replication initiation protein P exhibits a toxic effect on its Escherichia coli (E. coli) host, likely due to the formation of a dead-end P-DnaB complex, sequestering the replicative DnaB helicase from further activity. Intracellular expression of P triggers SOS-independent cellular filamentation and rapidly cures resident ColE1 plasmids. The toxicity of P is suppressed by alleles of P or dnaB. We asked whether P buildup within a cell can influence E. coli replication fidelity. The influence of P expression from a defective prophage, or when cloned and expressed from a plasmid was examined by screening for auxotrophic mutants, or by selection for rifampicin resistant (Rif(R)) cells acquiring mutations within the rpoB gene encoding the β-subunit of RNA polymerase ("],"journal":["Viruses"],"pubmed_title":["Lambda gpP-DnaB Helicase Sequestration and gpP-RpoB Associated Effects: On Screens for Auxotrophs, Selection for Rif(R), Toxicity, Mutagenicity, Plasmid Curing."],"pmcid":["PMC4926192"],"funding_grant_id":["138296-2012"],"pubmed_authors":["Chu A","Wang W","Hayes S","Rajamanickam K","Banerjee A","Hayes C"],"additional_accession":[]},"is_claimable":false,"name":"Lambda gpP-DnaB Helicase Sequestration and gpP-RpoB Associated Effects: On Screens for Auxotrophs, Selection for Rif(R), Toxicity, Mutagenicity, Plasmid Curing.","description":"The bacteriophage lambda replication initiation protein P exhibits a toxic effect on its Escherichia coli (E. coli) host, likely due to the formation of a dead-end P-DnaB complex, sequestering the replicative DnaB helicase from further activity. Intracellular expression of P triggers SOS-independent cellular filamentation and rapidly cures resident ColE1 plasmids. The toxicity of P is suppressed by alleles of P or dnaB. We asked whether P buildup within a cell can influence E. coli replication fidelity. The influence of P expression from a defective prophage, or when cloned and expressed from a plasmid was examined by screening for auxotrophic mutants, or by selection for rifampicin resistant (Rif(R)) cells acquiring mutations within the rpoB gene encoding the β-subunit of RNA polymerase (","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jun","modification":"2026-07-16T02:28:07.551Z","creation":"2019-03-27T02:17:09Z"},"accession":"S-EPMC4926192","cross_references":{"pubmed":["27338450"],"doi":["10.3390/v8060172"]}}