{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(6)"],"submitter":["Mitra A"],"pubmed_abstract":["The quorum sensing molecule Autoinducer-2 (AI-2) is generated as a byproduct of activated methyl cycle by the action of LuxS in Escherichia coli. AI-2 is synthesized, released and later internalized in a cell-density dependent manner. Here, by mutational analysis of the genes, uvrY and csrA, we describe a regulatory circuit of accumulation and uptake of AI-2. We constructed a single-copy chromosomal luxS-lacZ fusion in a luxS + merodiploid strain and evaluated its relative expression in uvrY and csrA mutants. At the entry of stationary phase, the expression of the fusion and AI-2 accumulation was positively regulated by uvrY and negatively regulated by csrA respectively. A deletion of csrA altered message stability of the luxS transcript and CsrA protein exhibited weak binding to 5' luxS r"],"journal":["PloS one"],"pagination":["e0157532"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4928848"],"repository":["biostudies-literature"],"pubmed_title":["Integration of AI-2 Based Cell-Cell Signaling with Metabolic Cues in Escherichia coli."],"pmcid":["PMC4928848"],"pubmed_authors":["Patel IR","Coleman A","Mitra A","Mukhopadhyay S","Herren CD"],"additional_accession":[]},"is_claimable":false,"name":"Integration of AI-2 Based Cell-Cell Signaling with Metabolic Cues in Escherichia coli.","description":"The quorum sensing molecule Autoinducer-2 (AI-2) is generated as a byproduct of activated methyl cycle by the action of LuxS in Escherichia coli. AI-2 is synthesized, released and later internalized in a cell-density dependent manner. Here, by mutational analysis of the genes, uvrY and csrA, we describe a regulatory circuit of accumulation and uptake of AI-2. We constructed a single-copy chromosomal luxS-lacZ fusion in a luxS + merodiploid strain and evaluated its relative expression in uvrY and csrA mutants. At the entry of stationary phase, the expression of the fusion and AI-2 accumulation was positively regulated by uvrY and negatively regulated by csrA respectively. A deletion of csrA altered message stability of the luxS transcript and CsrA protein exhibited weak binding to 5' luxS r","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2026-04-08T16:01:30.255Z","creation":"2026-04-08T05:56:26.72Z"},"accession":"S-EPMC4928848","cross_references":{"pubmed":["27362507"],"doi":["10.1371/journal.pone.0157532"]}}