{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Hu S"],"pubmed_abstract":["Heterologous expression has been proven to be a valid strategy for elucidating the natural products produced by gene clusters uncovered by genome sequencing projects. Efforts have been made to efficiently clone gene clusters directly from genomic DNA and several approaches have been developed. Here, we present an alternative strategy based on the site-specific recombinase system Cre/loxP for direct cloning gene clusters. A type three secretion system (T3SS) gene cluster (~32 kb) from Photorhabdus luminescens TT01 and DNA fragment (~78 kb) containing the siderophore biosynthetic gene cluster from Agrobacterium tumefaciens C58 have been successfully cloned into pBeloBAC11 with \"Cre/loxP plus BAC\" strategy. Based on the fact that Cre/loxP system has successfully used for genomic engineering i"],"journal":["Scientific reports"],"pagination":["29087"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4929569"],"repository":["biostudies-literature"],"pubmed_title":["\"Cre/loxP plus BAC\": a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens."],"pmcid":["PMC4929569"],"pubmed_authors":["Zhang G","Hu S","Liu Z","Ding X","Xie Y","Xia L","Zhang Y","Zhang X","Fu J","Stewart AF","Mo X"],"additional_accession":[]},"is_claimable":false,"name":"\"Cre/loxP plus BAC\": a strategy for direct cloning of large DNA fragment and its applications in Photorhabdus luminescens and Agrobacterium tumefaciens.","description":"Heterologous expression has been proven to be a valid strategy for elucidating the natural products produced by gene clusters uncovered by genome sequencing projects. Efforts have been made to efficiently clone gene clusters directly from genomic DNA and several approaches have been developed. Here, we present an alternative strategy based on the site-specific recombinase system Cre/loxP for direct cloning gene clusters. A type three secretion system (T3SS) gene cluster (~32 kb) from Photorhabdus luminescens TT01 and DNA fragment (~78 kb) containing the siderophore biosynthetic gene cluster from Agrobacterium tumefaciens C58 have been successfully cloned into pBeloBAC11 with \"Cre/loxP plus BAC\" strategy. Based on the fact that Cre/loxP system has successfully used for genomic engineering i","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jul","modification":"2026-05-05T14:57:33.584Z","creation":"2019-03-27T02:17:20Z"},"accession":"S-EPMC4929569","cross_references":{"pubmed":["27364376"],"doi":["10.1038/srep29087"]}}