{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nguyen AT"],"funding":["Wellcome Trust"],"pagination":["125"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4937578"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["<h4>Background</h4>Hand, foot and mouth disease (HFMD) has become a major public health problem across the Asia-Pacific region, and is commonly caused by enterovirus A71 (EV-A71) and coxsackievirus A6 (CV-A6), CV-A10 and CV-A16. Generating pathogen whole-genome sequences is essential for understanding their evolutionary biology. The frequent replacements among EV serotypes and a limited numbers of available whole-genome sequences hinder the development of overlapping PCRs for whole-genome sequencing. We developed and evaluated a non-ribosomal random PCR (rPCR) and next-generation sequencing based assay for sequence-independent whole-genome amplification and sequencing of HFMD pathogens. A total of 16 EV-A71/CV-A6/CV-A10/CV-A16 PCR positive rectal/throat swabs (Cp values: 20.9-33.3) were us"],"journal":["Virology journal"],"pubmed_title":["Development and evaluation of a non-ribosomal random PCR and next-generation sequencing based assay for detection and sequencing of hand, foot and mouth disease pathogens."],"pmcid":["PMC4937578"],"funding_grant_id":["089276/Z/09/Z","101104/Z/13/Z"],"pubmed_authors":["Ha TM","Nguyen AT","Nguyen CV","Ho VL","Tran TT","van Doorn HR","Phan QT","Nguyen HT","Hoang VM","Do VC","Thwaites G","Nghiem NM","Le TV","Truong KH","Le NN","Le TT"],"additional_accession":[]},"is_claimable":false,"name":"Development and evaluation of a non-ribosomal random PCR and next-generation sequencing based assay for detection and sequencing of hand, foot and mouth disease pathogens.","description":"<h4>Background</h4>Hand, foot and mouth disease (HFMD) has become a major public health problem across the Asia-Pacific region, and is commonly caused by enterovirus A71 (EV-A71) and coxsackievirus A6 (CV-A6), CV-A10 and CV-A16. Generating pathogen whole-genome sequences is essential for understanding their evolutionary biology. The frequent replacements among EV serotypes and a limited numbers of available whole-genome sequences hinder the development of overlapping PCRs for whole-genome sequencing. We developed and evaluated a non-ribosomal random PCR (rPCR) and next-generation sequencing based assay for sequence-independent whole-genome amplification and sequencing of HFMD pathogens. A total of 16 EV-A71/CV-A6/CV-A10/CV-A16 PCR positive rectal/throat swabs (Cp values: 20.9-33.3) were us","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jul","modification":"2026-06-17T06:03:44.95Z","creation":"2019-03-27T02:17:50Z"},"accession":"S-EPMC4937578","cross_references":{"pubmed":["27388326"],"doi":["10.1186/s12985-016-0580-9"]}}