biostudies-literatureAguado ABritish Heart FoundationNCI NIH HHSCanadian Institutes of Health Research253-65https://www.ebi.ac.uk/biostudies/studies/S-EPMC4947528biostudies-literatureUnknown34(2)<h4>Objective</h4>NOX-1 and NOX-4 are key enzymes responsible for reactive oxygen species (ROS) generation in vascular smooth muscle cells (VSMC). The RNA-binding protein Hu antigen R (HuR) is implicated in posttranscriptional regulation of gene expression; however, its role regulating NOX is unknown. We investigated transcriptional and posttranscriptional mechanisms underlying angiotensin II (AngII) and IL-1β regulation of NOX-1 and NOX-4 in VSMC and their implications in cell migration.<h4>Methods</h4>Rat and human VSMC were stimulated with AngII (0.1 μmol/l) and/or IL-1β (10 ng/ml). NOX-1 and NOX-4 mRNA and protein levels, NOX-1 and NOX-4 promoter and 3'UTR activities, NADPH oxidase activity, ROS production, and cell migration were studied.<h4>Results</h4>IL-1β increased NOX-1 expressioJournal of hypertensionHu antigen R is required for NOX-1 but not NOX-4 regulation by inflammatory stimuli in vascular smooth muscle cells.PMC4947528R01 CA134609R01CA134609RG/13/7/30099Salaices MFischer TMartinez-Gonzalez JManea AAguado ABriones AMRodriguez CAlonso MJDixon DAHernanz RTouyz RMfalseHu antigen R is required for NOX-1 but not NOX-4 regulation by inflammatory stimuli in vascular smooth muscle cells.<h4>Objective</h4>NOX-1 and NOX-4 are key enzymes responsible for reactive oxygen species (ROS) generation in vascular smooth muscle cells (VSMC). The RNA-binding protein Hu antigen R (HuR) is implicated in posttranscriptional regulation of gene expression; however, its role regulating NOX is unknown. We investigated transcriptional and posttranscriptional mechanisms underlying angiotensin II (AngII) and IL-1β regulation of NOX-1 and NOX-4 in VSMC and their implications in cell migration.<h4>Methods</h4>Rat and human VSMC were stimulated with AngII (0.1 μmol/l) and/or IL-1β (10 ng/ml). NOX-1 and NOX-4 mRNA and protein levels, NOX-1 and NOX-4 promoter and 3'UTR activities, NADPH oxidase activity, ROS production, and cell migration were studied.<h4>Results</h4>IL-1β increased NOX-1 expressio2016-01-01T00:00:00Z2016 Feb2025-04-03T23:47:42.708Z2019-03-27T02:18:32ZS-EPMC49475282668294210.1097/hjh.000000000000080110.1097/HJH.0000000000000801