{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mothersole DJ"],"funding":["European Research Council","U.S. Department of Energy, Office of Science","PARC's","BBSRC doctoral studentship","Photosynthetic Antenna Research Center (PARC)","Biotechnology and Biological Sciences Research Council (BBSRC UK)","Biotechnology and Biological Sciences Research Council"],"pagination":["307-27"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4949548"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["99(2)"],"pubmed_abstract":["The mature architecture of the photosynthetic membrane of the purple phototroph Rhodobacter sphaeroides has been characterised to a level where an atomic-level membrane model is available, but the roles of the putative assembly proteins LhaA and PucC in establishing this architecture are unknown. Here we investigate the assembly of light-harvesting LH2 and reaction centre-light-harvesting1-PufX (RC-LH1-PufX) photosystem complexes using spectroscopy, pull-downs, native gel electrophoresis, quantitative mass spectrometry and fluorescence lifetime microscopy to characterise a series of lhaA and pucC mutants. LhaA and PucC are important for specific assembly of LH1 or LH2 complexes, respectively, but they are not essential; the few LH1 subunits found in ΔlhaA mutants assemble to form normal RC"],"journal":["Molecular microbiology"],"pubmed_title":["PucC and LhaA direct efficient assembly of the light-harvesting complexes in Rhodobacter sphaeroides."],"pmcid":["PMC4949548"],"funding_grant_id":["BB/M000265/1","BB/G021546/1","338895","DE-SC 0001035"],"pubmed_authors":["Hunter CN","Brindley AA","Jackson PJ","Tucker JD","Vasilev C","Dickman MJ","Mothersole DJ"],"additional_accession":[]},"is_claimable":false,"name":"PucC and LhaA direct efficient assembly of the light-harvesting complexes in Rhodobacter sphaeroides.","description":"The mature architecture of the photosynthetic membrane of the purple phototroph Rhodobacter sphaeroides has been characterised to a level where an atomic-level membrane model is available, but the roles of the putative assembly proteins LhaA and PucC in establishing this architecture are unknown. Here we investigate the assembly of light-harvesting LH2 and reaction centre-light-harvesting1-PufX (RC-LH1-PufX) photosystem complexes using spectroscopy, pull-downs, native gel electrophoresis, quantitative mass spectrometry and fluorescence lifetime microscopy to characterise a series of lhaA and pucC mutants. LhaA and PucC are important for specific assembly of LH1 or LH2 complexes, respectively, but they are not essential; the few LH1 subunits found in ΔlhaA mutants assemble to form normal RC","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jan","modification":"2025-04-04T21:51:39.443Z","creation":"2019-03-27T02:18:41Z"},"accession":"S-EPMC4949548","cross_references":{"pubmed":["26419219"],"doi":["10.1111/mmi.13235"]}}