{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Eckl-Dorna J"],"funding":["Austrian Science Fund FWF"],"pagination":["1222-9"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4949646"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["70(10)"],"pubmed_abstract":["Background
The adaptive immunity underlying allergy comprises two components, the allergen-specific antibody (i.e. IgE, IgG) and the T-cell response. These two components are responsible for different disease manifestations and can be targeted by different therapeutic approaches. Here, we investigated the association of allergen-specific antibody and T- as well as B-cell responses in pollen-allergic patients using recombinant (r) major birch pollen allergen rBet v 1 and major timothy grass pollen allergen rPhl p 5 as defined antigens.Methods
Allergen-specific IgE and IgG antibody responses were determined by ELISA, and allergen-specific T- and B-cell responses were measured in peripheral blood mononuclear cells using a carboxyfluorescein-diacetate-succinimidylester (CFSE) d"],"journal":["Allergy"],"pubmed_title":["Poor association of allergen-specific antibody, T- and B-cell responses revealed with recombinant allergens and a CFSE dilution-based assay."],"pmcid":["PMC4949646"],"funding_grant_id":["F4613","F4605","F 4605"],"pubmed_authors":["Eckl-Dorna J","Valenta R","Campana R","Niederberger V"],"additional_accession":[]},"is_claimable":false,"name":"Poor association of allergen-specific antibody, T- and B-cell responses revealed with recombinant allergens and a CFSE dilution-based assay.","description":"Background
The adaptive immunity underlying allergy comprises two components, the allergen-specific antibody (i.e. IgE, IgG) and the T-cell response. These two components are responsible for different disease manifestations and can be targeted by different therapeutic approaches. Here, we investigated the association of allergen-specific antibody and T- as well as B-cell responses in pollen-allergic patients using recombinant (r) major birch pollen allergen rBet v 1 and major timothy grass pollen allergen rPhl p 5 as defined antigens.Methods
Allergen-specific IgE and IgG antibody responses were determined by ELISA, and allergen-specific T- and B-cell responses were measured in peripheral blood mononuclear cells using a carboxyfluorescein-diacetate-succinimidylester (CFSE) d","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Oct","modification":"2025-04-04T21:52:13.436Z","creation":"2019-03-27T02:18:42Z"},"accession":"S-EPMC4949646","cross_references":{"pubmed":["26043182"],"doi":["10.1111/all.12661"]}}