{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ma L"],"funding":["NIDDK NIH HHS"],"pagination":["857-64"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4954766"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(6)"],"pubmed_abstract":["Aim
Nanobody is an antibody fragment consisting of a single monomeric variable antibody domain, which can be used for a variety of biotechnological and therapeutic purposes. The aim of this work was to isolate and characterize a human signal domain antibody against VEGFR-2 domain3 (VEGFR D3) from a phage display library.Methods
To produce antigen-specific recombinant nanobodies with high affinity to VEGFR2 D3, a liquid phase panning strategy was used for all rounds of panning. For nanobody expression and purification, four VEGFR2 D3-blocking clones were subcloned into a pETduet-biotin-MBP expression vector. The recombinant proteins carried an MBP tag to facilitate purification by affinity chromatography. Recombinant NTV(1-4) was obtained after an additional gel filtration c"],"journal":["Acta pharmacologica Sinica"],"pubmed_title":["Generation and characterization of a human nanobody against VEGFR-2."],"pmcid":["PMC4954766"],"funding_grant_id":["R01 DK071662"],"pubmed_authors":["Jiang Y","Wang M","Ma L","Chen XT","Zhang J","Melcher K","Gu K","Zhang CH","Xu HE"],"additional_accession":[]},"is_claimable":false,"name":"Generation and characterization of a human nanobody against VEGFR-2.","description":"Aim
Nanobody is an antibody fragment consisting of a single monomeric variable antibody domain, which can be used for a variety of biotechnological and therapeutic purposes. The aim of this work was to isolate and characterize a human signal domain antibody against VEGFR-2 domain3 (VEGFR D3) from a phage display library.Methods
To produce antigen-specific recombinant nanobodies with high affinity to VEGFR2 D3, a liquid phase panning strategy was used for all rounds of panning. For nanobody expression and purification, four VEGFR2 D3-blocking clones were subcloned into a pETduet-biotin-MBP expression vector. The recombinant proteins carried an MBP tag to facilitate purification by affinity chromatography. Recombinant NTV(1-4) was obtained after an additional gel filtration c","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jun","modification":"2025-05-29T21:25:58.537Z","creation":"2025-05-29T21:25:58.537Z"},"accession":"S-EPMC4954766","cross_references":{"pubmed":["27108602"],"doi":["10.1038/aps.2016.2"]}}