biostudies-literatureMa LNIDDK NIH HHS857-64https://www.ebi.ac.uk/biostudies/studies/S-EPMC4954766biostudies-literatureUnknown37(6)<h4>Aim</h4>Nanobody is an antibody fragment consisting of a single monomeric variable antibody domain, which can be used for a variety of biotechnological and therapeutic purposes. The aim of this work was to isolate and characterize a human signal domain antibody against VEGFR-2 domain3 (VEGFR D3) from a phage display library.<h4>Methods</h4>To produce antigen-specific recombinant nanobodies with high affinity to VEGFR2 D3, a liquid phase panning strategy was used for all rounds of panning. For nanobody expression and purification, four VEGFR2 D3-blocking clones were subcloned into a pETduet-biotin-MBP expression vector. The recombinant proteins carried an MBP tag to facilitate purification by affinity chromatography. Recombinant NTV(1-4) was obtained after an additional gel filtration cActa pharmacologica SinicaGeneration and characterization of a human nanobody against VEGFR-2.PMC4954766R01 DK071662Jiang YWang MMa LChen XTZhang JMelcher KGu KZhang CHXu HEfalseGeneration and characterization of a human nanobody against VEGFR-2.<h4>Aim</h4>Nanobody is an antibody fragment consisting of a single monomeric variable antibody domain, which can be used for a variety of biotechnological and therapeutic purposes. The aim of this work was to isolate and characterize a human signal domain antibody against VEGFR-2 domain3 (VEGFR D3) from a phage display library.<h4>Methods</h4>To produce antigen-specific recombinant nanobodies with high affinity to VEGFR2 D3, a liquid phase panning strategy was used for all rounds of panning. For nanobody expression and purification, four VEGFR2 D3-blocking clones were subcloned into a pETduet-biotin-MBP expression vector. The recombinant proteins carried an MBP tag to facilitate purification by affinity chromatography. Recombinant NTV(1-4) was obtained after an additional gel filtration c2016-01-01T00:00:00Z2016 Jun2025-05-29T21:25:58.537Z2025-05-29T21:25:58.537ZS-EPMC49547662710860210.1038/aps.2016.2