{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jobling MG"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["ftw001"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4957749"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["74(3)"],"pubmed_abstract":["Heat-labile enterotoxins (LT) of enterotoxigenic Escherichia coli (ETEC) are structurally and functionally related to cholera toxin (CT). LT-I toxins are plasmid-encoded and flanked by IS elements, while LT-II toxins of type II ETEC are chromosomally encoded with flanking genes that appear phage related. Here, I determined the complete genomic sequence of the locus for the LT-IIa type strain SA53, and show that the LT-IIa genes are encoded by a 51 239 bp lambdoid prophage integrated at the rac locus, the site of a defective prophage in E. coli K12 strains. Of 50 LT-IIa and LT-IIc, 46 prophages also encode one member of two novel two-gene ADP-ribosyltransferase toxin families that are both related to pertussis toxin, which I named eplBA or ealAB, respectively. The eplBA and ealAB genes are "],"journal":["Pathogens and disease"],"pubmed_title":["The chromosomal nature of LT-II enterotoxins solved: a lambdoid prophage encodes both LT-II and one of two novel pertussis-toxin-like toxin family members in type II enterotoxigenic Escherichia coli."],"pmcid":["PMC4957749"],"funding_grant_id":["AI-31940","GM62116","R37 AI014107","AI-14107","GM098791"],"pubmed_authors":["Jobling MG"],"additional_accession":[]},"is_claimable":false,"name":"The chromosomal nature of LT-II enterotoxins solved: a lambdoid prophage encodes both LT-II and one of two novel pertussis-toxin-like toxin family members in type II enterotoxigenic Escherichia coli.","description":"Heat-labile enterotoxins (LT) of enterotoxigenic Escherichia coli (ETEC) are structurally and functionally related to cholera toxin (CT). LT-I toxins are plasmid-encoded and flanked by IS elements, while LT-II toxins of type II ETEC are chromosomally encoded with flanking genes that appear phage related. Here, I determined the complete genomic sequence of the locus for the LT-IIa type strain SA53, and show that the LT-IIa genes are encoded by a 51 239 bp lambdoid prophage integrated at the rac locus, the site of a defective prophage in E. coli K12 strains. Of 50 LT-IIa and LT-IIc, 46 prophages also encode one member of two novel two-gene ADP-ribosyltransferase toxin families that are both related to pertussis toxin, which I named eplBA or ealAB, respectively. The eplBA and ealAB genes are ","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Apr","modification":"2026-04-07T17:06:54.273Z","creation":"2019-03-27T02:18:59Z"},"accession":"S-EPMC4957749","cross_references":{"pubmed":["26755534"],"doi":["10.1093/femspd/ftw001"]}}