{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2(7)"],"submitter":["Geng Y"],"pubmed_abstract":["The emerging models of human embryonic stem cell (hESC) self-organizing organoids provide a valuable in vitro platform for studying self-organizing processes that presumably mimic in vivo human developmental events. Here we report that through a chemical screen, we identified two novel and structurally similar small molecules BIR1 and BIR2 which robustly induced the self-organization of a balloon-shaped three-dimensional structure when applied to two-dimensional adherent hESC cultures in the absence of growth factors. Gene expression analyses and functional assays demonstrated an endothelial identity of this balloon-like structure, while cell surface marker analyses revealed a VE-cadherin(+)CD31(+)CD34(+)KDR(+)CD43(-) putative endothelial progenitor population. Furthermore, molecular marker labeling and morphological examinations characterized several other distinct DiI-Ac-LDL(+) multi-cellular modules and a VEGFR3(+) sprouting structure in the balloon cultures that likely represented intermediate structures of balloon-formation."],"journal":["Heliyon"],"pagination":["e00133"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4971129"],"repository":["biostudies-literature"],"pubmed_title":["A small molecule-based strategy for endothelial differentiation and three-dimensional morphogenesis from human embryonic stem cells."],"pmcid":["PMC4971129"],"pubmed_authors":["Geng Y","Feng B"],"additional_accession":[]},"is_claimable":false,"name":"A small molecule-based strategy for endothelial differentiation and three-dimensional morphogenesis from human embryonic stem cells.","description":"The emerging models of human embryonic stem cell (hESC) self-organizing organoids provide a valuable in vitro platform for studying self-organizing processes that presumably mimic in vivo human developmental events. Here we report that through a chemical screen, we identified two novel and structurally similar small molecules BIR1 and BIR2 which robustly induced the self-organization of a balloon-shaped three-dimensional structure when applied to two-dimensional adherent hESC cultures in the absence of growth factors. Gene expression analyses and functional assays demonstrated an endothelial identity of this balloon-like structure, while cell surface marker analyses revealed a VE-cadherin(+)CD31(+)CD34(+)KDR(+)CD43(-) putative endothelial progenitor population. Furthermore, molecular marker labeling and morphological examinations characterized several other distinct DiI-Ac-LDL(+) multi-cellular modules and a VEGFR3(+) sprouting structure in the balloon cultures that likely represented intermediate structures of balloon-formation.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Jul","modification":"2025-04-18T19:46:12.432Z","creation":"2019-03-27T02:19:45Z"},"accession":"S-EPMC4971129","cross_references":{"pubmed":["27512727"],"doi":["10.1016/j.heliyon.2016.e00133"]}}