biostudies-literatureEdwards RKNCATS NIH HHSNIAID NIH HHSNIAMS NIH HHS3312-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC4975423biostudies-literatureUnknown29(20)<h4>Objective</h4>We sought to compare the skin microbiota above and below the panniculus of third-trimester pregnant women with class III obesity.<h4>Methods</h4>We collected swabs from the anterior panniculus and from the intertriginous area under the panniculus of women with class III obesity (body mass index ≥40 kg/m(2)) carrying a live singleton at ≥28 weeks. DNA was extracted. PCR with primers targeting the 16S rDNA V4 region were used to prepare an amplicon library. PCR products were sequenced (Illumina MiSeq platform). The 16S rDNA sequences were processed, integrated, analyzed and reported using QIIME and in-house developed scripts. Taxonomy was assigned using RDP Classifier (threshold 0.8) against the Greengenes 16S rDNA database.<h4>Results</h4>Twenty women were enrolled. One saThe journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal ObstetriciansGravidas with class III obesity: evaluating the abdominal skin microbiota above and below the panniculus (.).PMC4975423P30 AI027767UL1 TR000165P30 AR050948UL1 TR001417Szychowski JLefkowitz EJZhi DSubramaniam AEdwards RKMorrow CKumar RfalseGravidas with class III obesity: evaluating the abdominal skin microbiota above and below the panniculus (.).<h4>Objective</h4>We sought to compare the skin microbiota above and below the panniculus of third-trimester pregnant women with class III obesity.<h4>Methods</h4>We collected swabs from the anterior panniculus and from the intertriginous area under the panniculus of women with class III obesity (body mass index ≥40 kg/m(2)) carrying a live singleton at ≥28 weeks. DNA was extracted. PCR with primers targeting the 16S rDNA V4 region were used to prepare an amplicon library. PCR products were sequenced (Illumina MiSeq platform). The 16S rDNA sequences were processed, integrated, analyzed and reported using QIIME and in-house developed scripts. Taxonomy was assigned using RDP Classifier (threshold 0.8) against the Greengenes 16S rDNA database.<h4>Results</h4>Twenty women were enrolled. One sa2016-01-01T00:00:00Z2016 Oct2026-03-15T21:31:32.371Z2019-03-27T02:20:03ZS-EPMC49754232660027710.3109/14767058.2015.1124081