{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bhattacharya P"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["227-39"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4979537"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["38(1)"],"pubmed_abstract":["Activation-induced deaminase (AID) is the master regulator of class switch recombination (CSR) and somatic hypermutation (SHM), but the mechanisms regulating AID function are obscure. The differential pattern of switch plasmid activity in three IgM(+)/AID(+) and two IgG(+)/AID(+) B cell lines prompted an analysis of global gene expression to discover the origin of these cells. Gene profiling suggested that the IgG(+)/AID(+) B cell lines derived from germinal center B cells. Analysis of SHM potential demonstrates that the IgVkappa domains are inducibly diversified at high rate during in vitro culture. The mutation spectra focused to A:T base pairs, revealing a component of the hypermutation program that occurs preferentially during phase 2 of SHM. The A:T error spectra were analyzed and wer"],"journal":["European journal of immunology"],"pubmed_title":["Identification of murine B cell lines that undergo somatic hypermutation focused to A:T and G:C residues."],"pmcid":["PMC4979537"],"funding_grant_id":["GM52400","R01 AI052400"],"pubmed_authors":["Morse HC","Bhattacharya P","Grigera F","Rogozin IB","Kenter AL","McCarty T"],"additional_accession":[]},"is_claimable":false,"name":"Identification of murine B cell lines that undergo somatic hypermutation focused to A:T and G:C residues.","description":"Activation-induced deaminase (AID) is the master regulator of class switch recombination (CSR) and somatic hypermutation (SHM), but the mechanisms regulating AID function are obscure. The differential pattern of switch plasmid activity in three IgM(+)/AID(+) and two IgG(+)/AID(+) B cell lines prompted an analysis of global gene expression to discover the origin of these cells. Gene profiling suggested that the IgG(+)/AID(+) B cell lines derived from germinal center B cells. Analysis of SHM potential demonstrates that the IgVkappa domains are inducibly diversified at high rate during in vitro culture. The mutation spectra focused to A:T base pairs, revealing a component of the hypermutation program that occurs preferentially during phase 2 of SHM. The A:T error spectra were analyzed and wer","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Jan","modification":"2026-04-30T03:39:56.031Z","creation":"2026-04-07T15:46:21.816Z"},"accession":"S-EPMC4979537","cross_references":{"pubmed":["18081040"],"doi":["10.1002/eji.200737664"]}}