biostudies-literatureGordon SMNCATS NIH HHSNHLBI NIH HHSNational Mouse Metabolic Phenotyping CenterWSDGreat Rivers Affiliate of the American Heart AssociationNIH184-94https://www.ebi.ac.uk/biostudies/studies/S-EPMC4987197biostudies-literatureUnknown146<h4>Unlabelled</h4>Given their association with cardiovascular disease protection, there has been intense interest in understanding the biology of high density lipoproteins (HDL). HDL is actually a family of diverse particle types, each made up of discrete - but as yet undetermined - combinations of proteins drawn from up to 95 lipophilic plasma proteins. The abundant apolipoproteins (apo) of the A class (apoA-I, apoA-II and apoA-IV) have been proposed to act as organizing platforms for auxiliary proteins, but this concept has not been systematically evaluated. We assessed the impact of genetic knock down of each platform protein on the particle size distribution of auxiliary HDL proteins. Loss of apoA-I or apoA-II massively reduced HDL lipids and changed the plasma size pattern and/or abuJournal of proteomicsImpact of genetic deletion of platform apolipoproteins on the size distribution of the murine lipoproteome.PMC4987197R01 HL067093R21 HL104136R01 HL062542R01 HL111829HL67093HL111829UL1 TR001425HL104136K23 HL118132Li HLu LJTso PShah ASGordon SMZhu XDavidson WSReardon CAfalseImpact of genetic deletion of platform apolipoproteins on the size distribution of the murine lipoproteome.<h4>Unlabelled</h4>Given their association with cardiovascular disease protection, there has been intense interest in understanding the biology of high density lipoproteins (HDL). HDL is actually a family of diverse particle types, each made up of discrete - but as yet undetermined - combinations of proteins drawn from up to 95 lipophilic plasma proteins. The abundant apolipoproteins (apo) of the A class (apoA-I, apoA-II and apoA-IV) have been proposed to act as organizing platforms for auxiliary proteins, but this concept has not been systematically evaluated. We assessed the impact of genetic knock down of each platform protein on the particle size distribution of auxiliary HDL proteins. Loss of apoA-I or apoA-II massively reduced HDL lipids and changed the plasma size pattern and/or abu2016-01-01T00:00:00Z2016 Sep2025-04-25T20:26:52.005Z2019-03-27T02:20:47ZS-EPMC49871972738537510.1016/j.jprot.2016.06.035