{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Boxman J"],"pubmed_abstract":["Embryonic stem cells can spontaneously differentiate into cell types of all germ layers within embryoid bodies (EBs) in a highly variable manner. Whether there exists an intrinsic differentiation program common to all EBs is unknown. Here, we present a novel combination of high-throughput live two-photon imaging and gene expression profiling to study early differentiation dynamics spontaneously occurring within developing EBs. Onset timing of Brachyury-GFP was highly variable across EBs, while the spatial patterns as well as the dynamics of mesendodermal progression following onset were remarkably similar. We therefore defined a 'developmental clock' using the Brachyury-GFP signal onset timing. Mapping snapshot gene expression measurements to this clock revealed their temporal trends, indi"],"journal":["Scientific reports"],"pagination":["31623"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4987683"],"repository":["biostudies-literature"],"pubmed_title":["Integrated live imaging and molecular profiling of embryoid bodies reveals a synchronized progression of early differentiation."],"pmcid":["PMC4987683"],"pubmed_authors":["Vadigepalli R","Nachman I","Achanta S","Sagy N","Boxman J"],"additional_accession":[]},"is_claimable":false,"name":"Integrated live imaging and molecular profiling of embryoid bodies reveals a synchronized progression of early differentiation.","description":"Embryonic stem cells can spontaneously differentiate into cell types of all germ layers within embryoid bodies (EBs) in a highly variable manner. Whether there exists an intrinsic differentiation program common to all EBs is unknown. Here, we present a novel combination of high-throughput live two-photon imaging and gene expression profiling to study early differentiation dynamics spontaneously occurring within developing EBs. Onset timing of Brachyury-GFP was highly variable across EBs, while the spatial patterns as well as the dynamics of mesendodermal progression following onset were remarkably similar. We therefore defined a 'developmental clock' using the Brachyury-GFP signal onset timing. Mapping snapshot gene expression measurements to this clock revealed their temporal trends, indi","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Aug","modification":"2025-04-04T10:35:58.851Z","creation":"2019-03-27T02:20:50Z"},"accession":"S-EPMC4987683","cross_references":{"pubmed":["27530599"],"doi":["10.1038/srep31623"]}}