{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu H"],"funding":["Human Frontier Science Program","University of Melbourne","NHMRC","National Health and Medical Research Council","Australian Research Council"],"pagination":["1695-703"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC4995085"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["213(9)"],"pubmed_abstract":["Major histocompatibility complex class II (MHC II) expression is tightly regulated, being subjected to cell type-specific mechanisms that closely control its levels at the cell surface. Ubiquitination by the E3 ubiquitin ligase MARCH 1 regulates MHC II expression in dendritic cells and B cells. In this study, we demonstrate that the related ligase MARCH 8 is responsible for regulating surface MHC II in thymic epithelial cells (TECs). March8(-/-) mice have elevated MHC II at the surface of cortical TECs and autoimmune regulator (AIRE)(-) medullary TECs (mTECs), but not AIRE(+) mTECs. Despite this, thymic and splenic CD4(+) T cell numbers and repertoires remained unaltered in March8(-/-) mice. Notably, the ubiquitination of MHC II by MARCH 8 is controlled by CD83. Mice expressing a mutated f"],"journal":["The Journal of experimental medicine"],"pubmed_title":["Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface MHC II expression in thymic epithelium and CD4 T cell selection."],"pmcid":["PMC4995085"],"funding_grant_id":["1049724","1058193","DP110101383","1016629","1090236","RGP0064","1071916","1078763"],"pubmed_authors":["Liu H","Jain R","Gray DH","Villadangos JA","Vuong V","Guan J","La Gruta NL","Mintern JD","Ishido S"],"additional_accession":[]},"is_claimable":false,"name":"Ubiquitin ligase MARCH 8 cooperates with CD83 to control surface MHC II expression in thymic epithelium and CD4 T cell selection.","description":"Major histocompatibility complex class II (MHC II) expression is tightly regulated, being subjected to cell type-specific mechanisms that closely control its levels at the cell surface. Ubiquitination by the E3 ubiquitin ligase MARCH 1 regulates MHC II expression in dendritic cells and B cells. In this study, we demonstrate that the related ligase MARCH 8 is responsible for regulating surface MHC II in thymic epithelial cells (TECs). March8(-/-) mice have elevated MHC II at the surface of cortical TECs and autoimmune regulator (AIRE)(-) medullary TECs (mTECs), but not AIRE(+) mTECs. Despite this, thymic and splenic CD4(+) T cell numbers and repertoires remained unaltered in March8(-/-) mice. Notably, the ubiquitination of MHC II by MARCH 8 is controlled by CD83. Mice expressing a mutated f","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Aug","modification":"2025-04-25T22:39:36.891Z","creation":"2019-03-27T02:22:28Z"},"accession":"S-EPMC4995085","cross_references":{"pubmed":["27503069"],"doi":["10.1084/jem.20160312"]}}