{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Munoz-Amatriain M"],"funding":["NSF Plant Genome Research Program","Office of Science of the US Department of Energy","UC Riverside Agricultural Experiment Station Hatch Project","NSF-ABI","USDA Initiative for Future Agriculture and Food Systems","North American Barley Genome Project","National Program of Sustainability I","USDA-CSREES National Research Initiative (NRI)","BarleyCAP","TriticeaeCAP"],"pagination":["216-27"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5014227"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["84(1)"],"pubmed_abstract":["Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although dis"],"journal":["The Plant journal : for cell and molecular biology"],"pubmed_title":["Sequencing of 15 622 gene-bearing BACs clarifies the gene-dense regions of the barley genome."],"pmcid":["PMC5014227"],"funding_grant_id":["CA-R-BPS-5306-H","USDA-NIFA 2010-15718-10","DE-AC02-05CH11231","USDA-CSREES-NRI 2006-55606-16722","DBI-0321756","01-52100-11346","DBI-1062301","LO1204","2002-35300-12548","USDA-AFRI-NIFA 2009-85606-05701","USDA-AFRI-NIFA 2009-65300-05645","USDA-CSREES 2001-34213-10511"],"pubmed_authors":["Cooper L","Dilbirligi M","Heinen S","Bozdag S","Simkova H","Dolezel J","Madishetty K","Kleinhofs A","Wu Y","Muehlbauer GJ","Moscou MJ","Schmutz J","Condamine P","Mirebrahim H","Beccuti M","Bhat PR","Ounit R","Graner A","Lemaux P","Close TJ","Duma D","Falk A","Lonardi S","Resnik J","Feiz L","Ma Y","Rodriguez E","Jiang T","Stein N","Zheng J","Wanamaker S","Chao S","Grimwood J","Alpert M","Kudrna D","Hayes PM","Munoz-Amatriain M","Svensson JT","Blake T","Wing R","Mammadov J","Bregitzer P","Cordero F","Luo M","Witt HN","Wise RP","Altschmied L","Gustafson P","You F"],"additional_accession":[]},"is_claimable":false,"name":"Sequencing of 15 622 gene-bearing BACs clarifies the gene-dense regions of the barley genome.","description":"Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although dis","dates":{"release":"2015-01-01T00:00:00Z","publication":"2015 Oct","modification":"2026-04-30T03:42:33.298Z","creation":"2019-03-27T02:23:41Z"},"accession":"S-EPMC5014227","cross_references":{"pubmed":["26252423"],"doi":["10.1111/tpj.12959"]}}