biostudies-literatureMunoz-Amatriain MNSF Plant Genome Research ProgramOffice of Science of the US Department of EnergyUC Riverside Agricultural Experiment Station Hatch ProjectNSF-ABIUSDA Initiative for Future Agriculture and Food SystemsNorth American Barley Genome ProjectNational Program of Sustainability IUSDA-CSREES National Research Initiative (NRI)BarleyCAPTriticeaeCAP216-27https://www.ebi.ac.uk/biostudies/studies/S-EPMC5014227biostudies-literatureUnknown84(1)Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although disThe Plant journal : for cell and molecular biologySequencing of 15 622 gene-bearing BACs clarifies the gene-dense regions of the barley genome.PMC5014227CA-R-BPS-5306-HUSDA-NIFA 2010-15718-10DE-AC02-05CH11231USDA-CSREES-NRI 2006-55606-16722DBI-032175601-52100-11346DBI-1062301LO12042002-35300-12548USDA-AFRI-NIFA 2009-85606-05701USDA-AFRI-NIFA 2009-65300-05645USDA-CSREES 2001-34213-10511Cooper LDilbirligi MHeinen SBozdag SSimkova HDolezel JMadishetty KKleinhofs AWu YMuehlbauer GJMoscou MJSchmutz JCondamine PMirebrahim HBeccuti MBhat PROunit RGraner ALemaux PClose TJDuma DFalk ALonardi SResnik JFeiz LMa YRodriguez EJiang TStein NZheng JWanamaker SChao SGrimwood JAlpert MKudrna DHayes PMMunoz-Amatriain MSvensson JTBlake TWing RMammadov JBregitzer PCordero FLuo MWitt HNWise RPAltschmied LGustafson PYou FfalseSequencing of 15 622 gene-bearing BACs clarifies the gene-dense regions of the barley genome.Barley (Hordeum vulgare L.) possesses a large and highly repetitive genome of 5.1 Gb that has hindered the development of a complete sequence. In 2012, the International Barley Sequencing Consortium released a resource integrating whole-genome shotgun sequences with a physical and genetic framework. However, because only 6278 bacterial artificial chromosome (BACs) in the physical map were sequenced, fine structure was limited. To gain access to the gene-containing portion of the barley genome at high resolution, we identified and sequenced 15 622 BACs representing the minimal tiling path of 72 052 physical-mapped gene-bearing BACs. This generated ~1.7 Gb of genomic sequence containing an estimated 2/3 of all Morex barley genes. Exploration of these sequenced BACs revealed that although dis2015-01-01T00:00:00Z2015 Oct2026-04-30T03:42:33.298Z2019-03-27T02:23:41ZS-EPMC50142272625242310.1111/tpj.12959