biostudies-literatureUnknown11(9)Mendonca Belmont TF<h4>Introduction</h4>Patients with sickle cell anemia (SCA) may present chronic hemolytic anemia, vaso-occlusion and respiratory tract infection (RTI) episodes. Galectin-3 (GAL-3) is a multifunctional protein involved in inflammation, apoptosis, adhesion and resistance to reactive oxygen species. Studies point to a dual role for GAL-3 as both a circulation damage-associated molecular pattern and a cell membrane associated pattern recognition receptor.<h4>Objective</h4>To investigate associations between the SNPs of GAL-3 gene (LGALS3) and serum levels with RTI and vaso-occlusive crisis (VOC) in children with SCA.<h4>Materials and methods</h4>SNPs +191 and +292 in LGALS3 were studied using the TaqMan real-time PCR system; GAL-3 serum levels were measured by ELISA. The study included 79 chilPloS onee0162297https://www.ebi.ac.uk/biostudies/studies/S-EPMC5014331biostudies-literatureSingle Nucleotide Polymorphisms at +191 and +292 of Galectin-3 Gene (LGALS3) Related to Lower GAL-3 Serum Levels Are Associated with Frequent Respiratory Tract Infection and Vaso-Occlusive Crisis in Children with Sickle Cell Anemia.PMC5014331Araujo Ada SCavalcanti Mdo SSilva Vasconcelos LRSilva Medeiros FSoares da Silva Ado O KPBezerra MAMendonca Dos Anjos ACde Melo Rego MJDomingues Hatzlhofer BLde Melo Vilar KMoura PMendonca Belmont TFPitta MGfalseSingle Nucleotide Polymorphisms at +191 and +292 of Galectin-3 Gene (LGALS3) Related to Lower GAL-3 Serum Levels Are Associated with Frequent Respiratory Tract Infection and Vaso-Occlusive Crisis in Children with Sickle Cell Anemia.<h4>Introduction</h4>Patients with sickle cell anemia (SCA) may present chronic hemolytic anemia, vaso-occlusion and respiratory tract infection (RTI) episodes. Galectin-3 (GAL-3) is a multifunctional protein involved in inflammation, apoptosis, adhesion and resistance to reactive oxygen species. Studies point to a dual role for GAL-3 as both a circulation damage-associated molecular pattern and a cell membrane associated pattern recognition receptor.<h4>Objective</h4>To investigate associations between the SNPs of GAL-3 gene (LGALS3) and serum levels with RTI and vaso-occlusive crisis (VOC) in children with SCA.<h4>Materials and methods</h4>SNPs +191 and +292 in LGALS3 were studied using the TaqMan real-time PCR system; GAL-3 serum levels were measured by ELISA. The study included 79 chil2016-01-01T00:00:00Z20162026-04-30T03:42:39.016Z2019-03-26T22:54:12ZS-EPMC50143312760370310.1371/journal.pone.0162297