biostudies-literatureUnknown31(3)Ishii SPseudogulbenkiania is a relatively recently characterized genus within the order Neisseriales, class Betaproteobacteria. This genus contains several strains that are capable of anaerobic, nitrate-dependent Fe(II) oxidation (NDFO), a geochemically important reaction for nitrogen and iron cycles. In the present study, we examined denitrification functional gene diversities within this genus, and clarified whether other Pseudogulbenkiania sp. strains perform denitrification and NDFO. Seventy strains were analyzed, including two type strains, a well-characterized NDFO strain, and 67 denitrifying strains isolated from various rice paddy fields and rice-soybean rotation fields in Japan. We also attempted to identify the genes responsible for NDFO by mutagenesis. Our comprehensive analysis showedMicrobes and environments293-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC5017806biostudies-literatureDenitrification and Nitrate-Dependent Fe(II) Oxidation in Various Pseudogulbenkiania Strains.PMC5017806Joikai KSenoo KOkabe SIshii SOtsuka SfalseDenitrification and Nitrate-Dependent Fe(II) Oxidation in Various Pseudogulbenkiania Strains.Pseudogulbenkiania is a relatively recently characterized genus within the order Neisseriales, class Betaproteobacteria. This genus contains several strains that are capable of anaerobic, nitrate-dependent Fe(II) oxidation (NDFO), a geochemically important reaction for nitrogen and iron cycles. In the present study, we examined denitrification functional gene diversities within this genus, and clarified whether other Pseudogulbenkiania sp. strains perform denitrification and NDFO. Seventy strains were analyzed, including two type strains, a well-characterized NDFO strain, and 67 denitrifying strains isolated from various rice paddy fields and rice-soybean rotation fields in Japan. We also attempted to identify the genes responsible for NDFO by mutagenesis. Our comprehensive analysis showed2016-01-01T00:00:00Z2016 Sep2026-05-04T15:07:33.569Z2019-03-27T02:23:52ZS-EPMC50178062743137310.1264/jsme2.ME16001