{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Paulo JA"],"funding":["National Institute of Diabetes and Digestive and Kidney Diseases","NIDDK NIH HHS","National Institute of General Medical Sciences"],"pagination":["1620-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5018445"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(10)"],"pubmed_abstract":["Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. When properly implemented, synchronous precursor selection and triple-stage mass spectrometry (SPS-MS3) can reduce the occurrence of this phenomenon, referred to as ion interference. However, no diagnostic tool is available currently to rapidly and accurately assess ion interference. To address this need, we developed a multiplexed tandem mass tag (TMT)-based standard, termed the triple knockout (TKO). This standard is comprised of three yeast proteomes in triplicate, each from a strain deficien"],"journal":["Journal of the American Society for Mass Spectrometry"],"pubmed_title":["A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments."],"pmcid":["PMC5018445"],"funding_grant_id":["K01 DK098285","DK098285","GM97645"],"pubmed_authors":["O'Connell JD","Paulo JA","Gygi SP"],"additional_accession":[]},"is_claimable":false,"name":"A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments.","description":"Isobaric labeling is a powerful strategy for quantitative mass spectrometry-based proteomic investigations. A complication of such analyses has been the co-isolation of multiple analytes of similar mass-to-charge resulting in the distortion of relative protein abundance measurements across samples. When properly implemented, synchronous precursor selection and triple-stage mass spectrometry (SPS-MS3) can reduce the occurrence of this phenomenon, referred to as ion interference. However, no diagnostic tool is available currently to rapidly and accurately assess ion interference. To address this need, we developed a multiplexed tandem mass tag (TMT)-based standard, termed the triple knockout (TKO). This standard is comprised of three yeast proteomes in triplicate, each from a strain deficien","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Oct","modification":"2025-04-19T07:40:32.488Z","creation":"2019-03-27T02:23:54Z"},"accession":"S-EPMC5018445","cross_references":{"pubmed":["27400695"],"doi":["10.1007/s13361-016-1434-9"]}}