{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Zhou Q"],"pubmed_abstract":["<i>Haemophilus parasuis</i> is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of <i>H. parasuis</i> is a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis in <i>H. parasuis</i> SC096 were identified (<i>lgtB</i> and <i>lex-1</i>). Mutants were constructed to investigate the roles of the <i>lgtB</i> and <i>lex-1</i> genes. The LOS from the Δ<i>lgtB</i> or Δ<i>lex-1</i> mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain. The Δ<i>lgtB</i> and Δ<i>lex-1</i> mutants were significantly more sensitive to 50% porcine serum, displaying 15.0 and 54.46% survival rates, respectively. C"],"journal":["Frontiers in cellular and infection microbiology"],"pagination":["100"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5018477"],"repository":["biostudies-literature"],"pubmed_title":["Two Glycosyltransferase Genes of <i>Haemophilus parasuis</i> SC096 Implicated in Lipooligosaccharide Biosynthesis, Serum Resistance, Adherence, and Invasion."],"pmcid":["PMC5018477"],"pubmed_authors":["Jia A","Xing K","Fan H","Zhou Q","Zhang J","Yang K","Feng S","Liao M"],"additional_accession":[]},"is_claimable":false,"name":"Two Glycosyltransferase Genes of <i>Haemophilus parasuis</i> SC096 Implicated in Lipooligosaccharide Biosynthesis, Serum Resistance, Adherence, and Invasion.","description":"<i>Haemophilus parasuis</i> is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of <i>H. parasuis</i> is a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis in <i>H. parasuis</i> SC096 were identified (<i>lgtB</i> and <i>lex-1</i>). Mutants were constructed to investigate the roles of the <i>lgtB</i> and <i>lex-1</i> genes. The LOS from the Δ<i>lgtB</i> or Δ<i>lex-1</i> mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain. The Δ<i>lgtB</i> and Δ<i>lex-1</i> mutants were significantly more sensitive to 50% porcine serum, displaying 15.0 and 54.46% survival rates, respectively. C","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2026-04-08T01:55:38.212Z","creation":"2019-03-27T02:23:54Z"},"accession":"S-EPMC5018477","cross_references":{"pubmed":["27672622"],"doi":["10.3389/fcimb.2016.00100"]}}