biostudies-literatureUnknown6Zhou Q<i>Haemophilus parasuis</i> is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of <i>H. parasuis</i> is a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis in <i>H. parasuis</i> SC096 were identified (<i>lgtB</i> and <i>lex-1</i>). Mutants were constructed to investigate the roles of the <i>lgtB</i> and <i>lex-1</i> genes. The LOS from the Δ<i>lgtB</i> or Δ<i>lex-1</i> mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain. The Δ<i>lgtB</i> and Δ<i>lex-1</i> mutants were significantly more sensitive to 50% porcine serum, displaying 15.0 and 54.46% survival rates, respectively. CFrontiers in cellular and infection microbiology100https://www.ebi.ac.uk/biostudies/studies/S-EPMC5018477biostudies-literatureTwo Glycosyltransferase Genes of <i>Haemophilus parasuis</i> SC096 Implicated in Lipooligosaccharide Biosynthesis, Serum Resistance, Adherence, and Invasion.PMC5018477Jia AXing KFan HZhou QZhang JYang KFeng SLiao MfalseTwo Glycosyltransferase Genes of <i>Haemophilus parasuis</i> SC096 Implicated in Lipooligosaccharide Biosynthesis, Serum Resistance, Adherence, and Invasion.<i>Haemophilus parasuis</i> is a common opportunistic pathogen known for its ability to colonize healthy piglets and causes Glässer's disease. The lipooligosaccharide (LOS) of <i>H. parasuis</i> is a potential virulence-associated factor. In this study, two putative glycosyltransferases that might be involved in LOS synthesis in <i>H. parasuis</i> SC096 were identified (<i>lgtB</i> and <i>lex-1</i>). Mutants were constructed to investigate the roles of the <i>lgtB</i> and <i>lex-1</i> genes. The LOS from the Δ<i>lgtB</i> or Δ<i>lex-1</i> mutant showed truncated structure on silver-stained SDS-PAGE gel compared to the wild-type strain. The Δ<i>lgtB</i> and Δ<i>lex-1</i> mutants were significantly more sensitive to 50% porcine serum, displaying 15.0 and 54.46% survival rates, respectively. C2016-01-01T00:00:00Z20162026-04-08T01:55:38.212Z2019-03-27T02:23:54ZS-EPMC50184772767262210.3389/fcimb.2016.00100