biostudies-literatureLambert Emo KNational Institute of Allergy and Infectious DiseasesNIAID NIH HHSe1005881https://www.ebi.ac.uk/biostudies/studies/S-EPMC5028057biostudies-literatureUnknown12(9)During a primary influenza infection, cytotoxic CD8+ T cells need to infiltrate the infected airways and engage virus-infected epithelial cells. The factors that regulate T cell motility in the infected airway tissue are not well known. To more precisely study T cell infiltration of the airways, we developed an experimental model system using the trachea as a site where live imaging can be performed. CD8+ T cell motility was dynamic with marked changes in motility on different days of the infection. In particular, significant changes in average cell velocity and confinement were evident on days 8-10 during which the T cells abruptly but transiently increase velocity on day 9. Experiments to distinguish whether infection itself or antigen affect motility revealed that it is antigen, not actPLoS pathogensLive Imaging of Influenza Infection of the Trachea Reveals Dynamic Regulation of CD8+ T Cell Motility by Antigen.PMC50280571P01 AI102851-O1A1P01 AI102851HHSN272201200005CHHSN272201400005CBarilla CFowell DHyun YMTopham DJLambert Emo KGerber SKim MReilly EfalseLive Imaging of Influenza Infection of the Trachea Reveals Dynamic Regulation of CD8+ T Cell Motility by Antigen.During a primary influenza infection, cytotoxic CD8+ T cells need to infiltrate the infected airways and engage virus-infected epithelial cells. The factors that regulate T cell motility in the infected airway tissue are not well known. To more precisely study T cell infiltration of the airways, we developed an experimental model system using the trachea as a site where live imaging can be performed. CD8+ T cell motility was dynamic with marked changes in motility on different days of the infection. In particular, significant changes in average cell velocity and confinement were evident on days 8-10 during which the T cells abruptly but transiently increase velocity on day 9. Experiments to distinguish whether infection itself or antigen affect motility revealed that it is antigen, not act2016-01-01T00:00:00Z2016 Sep2025-04-05T15:57:18.789Z2019-03-26T22:45:03ZS-EPMC50280572764408910.1371/journal.ppat.1005881