biostudies-literatureUnknown157(10)Wodarski RBurrowing, an ethologically relevant rodent behaviour, has been proposed as a novel outcome measure to assess the global impact of pain in rats. In a prospective multicentre study using male rats (Wistar, Sprague-Dawley), replication of suppressed burrowing behaviour in the complete Freund adjuvant (CFA)-induced model of inflammatory pain (unilateral, 1 mg/mL in 100 µL) was evaluated in 11 studies across 8 centres. Following a standard protocol, data from participating centres were collected centrally and analysed with a restricted maximum likelihood-based mixed model for repeated measures. The total population (TP-all animals allocated to treatment; n = 249) and a selected population (SP-TP animals burrowing over 500 g at baseline; n = 200) were analysed separately, assessing the effect oPain2350-65https://www.ebi.ac.uk/biostudies/studies/S-EPMC5028161biostudies-literatureCross-centre replication of suppressed burrowing behaviour as an ethologically relevant pain outcome measure in the rat: a prospective multicentre study.PMC5028161Caspani OPekcec ASvensson CIKoyama SDelaney ABaastrup CGardiner NJBryden LAAndrews NLigocki MMorland RRotariu SMKennedy JDUto KLindsten ASegerdahl MStenfors CYamamoto KWodarski RVo SLi DChristoph TRutten KTreede RDRice ASUltenius CHuang WMachin IRobens AfalseCross-centre replication of suppressed burrowing behaviour as an ethologically relevant pain outcome measure in the rat: a prospective multicentre study.Burrowing, an ethologically relevant rodent behaviour, has been proposed as a novel outcome measure to assess the global impact of pain in rats. In a prospective multicentre study using male rats (Wistar, Sprague-Dawley), replication of suppressed burrowing behaviour in the complete Freund adjuvant (CFA)-induced model of inflammatory pain (unilateral, 1 mg/mL in 100 µL) was evaluated in 11 studies across 8 centres. Following a standard protocol, data from participating centres were collected centrally and analysed with a restricted maximum likelihood-based mixed model for repeated measures. The total population (TP-all animals allocated to treatment; n = 249) and a selected population (SP-TP animals burrowing over 500 g at baseline; n = 200) were analysed separately, assessing the effect o2016-01-01T00:00:00Z2016 Oct2026-05-05T08:37:32.75Z2026-04-07T21:35:37.723ZS-EPMC50281612764383610.1097/j.pain.0000000000000657