biostudies-literatureSahl JWDOD | Defense Threat Reduction Agencye00846-16https://www.ebi.ac.uk/biostudies/studies/S-EPMC5030356biostudies-literatureUnknown7(5)<h4>Unlabelled</h4>Whole-genome sequence (WGS) data are commonly used to design diagnostic targets for the identification of bacterial pathogens. To do this effectively, genomics databases must be comprehensive to identify the strict core genome that is specific to the target pathogen. As additional genomes are analyzed, the core genome size is reduced and there is erosion of the target-specific regions due to commonality with related species, potentially resulting in the identification of false positives and/or false negatives.<h4>Importance</h4>A comparative analysis of 1,130 Burkholderia genomes identified unique markers for many named species, including the human pathogens B. pseudomallei and B. mallei Due to core genome reduction and signature erosion, only 38 targets specific to B. pmBioThe Effects of Signal Erosion and Core Genome Reduction on the Identification of Diagnostic Markers.PMC5030356HDTRA1-12-C-0066CB10246Mayo MTuanyok AShippy KWuthiekanun VWagner DMPearson TWongsuwan GSarovich DSLovett SLimmathurotsakul DLadner JBusch JDVazquez AJSchupp JMKoroleva GAllender CJHutcheson ALummis MSahl JWCurrie BJColman REPrice EPKeim PKorlach JLiPuma JJHall CMTheobald VPalacios GfalseThe Effects of Signal Erosion and Core Genome Reduction on the Identification of Diagnostic Markers.<h4>Unlabelled</h4>Whole-genome sequence (WGS) data are commonly used to design diagnostic targets for the identification of bacterial pathogens. To do this effectively, genomics databases must be comprehensive to identify the strict core genome that is specific to the target pathogen. As additional genomes are analyzed, the core genome size is reduced and there is erosion of the target-specific regions due to commonality with related species, potentially resulting in the identification of false positives and/or false negatives.<h4>Importance</h4>A comparative analysis of 1,130 Burkholderia genomes identified unique markers for many named species, including the human pathogens B. pseudomallei and B. mallei Due to core genome reduction and signature erosion, only 38 targets specific to B. p2016-01-01T00:00:00Z2016 Sep2026-04-07T17:16:48.792Z2019-03-27T02:24:46ZS-EPMC50303562765135710.1128/mBio.00846-16