biostudies-literatureLaks DRNational Institutes of Neurological Disorders and Stroke (NINDS)NCATS NIH HHSNICHD NIH HHSCalifornia Institute of Regenerative Medicine (CIRM) Training GrantNINDS NIH HHSNCI NIH HHSNINDS1367-78https://www.ebi.ac.uk/biostudies/studies/S-EPMC5035518biostudies-literatureUnknown18(10)<h4>Background</h4>Gliomasphere cultures are widely utilized for the study of glioblastoma (GBM). However, this model system is not well characterized, and the utility of current classification methods is not clear.<h4>Methods</h4>We used 71 gliomasphere cultures from 68 individuals. Using gene expression-based classification, we performed unsupervised clustering and associated gene expression with gliomasphere phenotypes and patient survival.<h4>Results</h4>Some aspects of the gene expression-based classification method were robust because the gliomasphere cultures retained their classification over many passages, and IDH1 mutant gliomaspheres were all proneural. While gene expression of a subset of gliomasphere cultures was more like the parent tumor than any other tumor, gliomaspheres dNeuro-oncologyLarge-scale assessment of the gliomasphere model system.PMC5035518R56 NS052563R25 NS079198TG2-01169NS052563P30 CA016042R01 CA179071UL1 TR000124R01 NS052563P30 NS062691U54 HD087101Garrett MCLiau LMCloughesy TFLaks DRLai ASperry JShih MYGao FCoppola GMottahedeh JYong WHCrisman TJKornblum HIfalseLarge-scale assessment of the gliomasphere model system.<h4>Background</h4>Gliomasphere cultures are widely utilized for the study of glioblastoma (GBM). However, this model system is not well characterized, and the utility of current classification methods is not clear.<h4>Methods</h4>We used 71 gliomasphere cultures from 68 individuals. Using gene expression-based classification, we performed unsupervised clustering and associated gene expression with gliomasphere phenotypes and patient survival.<h4>Results</h4>Some aspects of the gene expression-based classification method were robust because the gliomasphere cultures retained their classification over many passages, and IDH1 mutant gliomaspheres were all proneural. While gene expression of a subset of gliomasphere cultures was more like the parent tumor than any other tumor, gliomaspheres d2016-01-01T00:00:00Z2016 Oct2026-05-05T05:43:05.37Z2019-03-27T02:25:03ZS-EPMC50355182711697810.1093/neuonc/now045