biostudies-literatureSengupta ANHGRI NIH HHSNCI NIH HHSNINDS NIH HHSE23https://www.ebi.ac.uk/biostudies/studies/S-EPMC5041122biostudies-literatureUnknown6(3)Oscillations in circadian metabolism are crucial to the well being of organism. Our understanding of metabolic rhythms has been greatly enhanced by recent advances in high-throughput systems biology experimental techniques and data analysis. In an in vitro setting, metabolite rhythms can be measured by time-dependent sampling over an experimental period spanning one or more days at sufficent resolution to elucidate rhythms. We hypothesized that cellular metabolic effects over such a time course would be influenced by both oscillatory and circadian-independent cell metabolic effects. Here we use nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling of mammalian cell culture media of synchronized U2 OS cells containing an intact transcriptional clock. The experiment was conMetabolitesDeciphering the Duality of Clock and Growth Metabolism in a Cell Autonomous System Using NMR Profiling of the Secretome.PMC5041122R01 CA051497R01 NS054794T32 HG000046Van Dang CWeljie AMOlarerin-George AOKrishnaiah SYGrowe JVenkataraman ARhoades SSlaff BSengupta AHogenesch JBfalseDeciphering the Duality of Clock and Growth Metabolism in a Cell Autonomous System Using NMR Profiling of the Secretome.Oscillations in circadian metabolism are crucial to the well being of organism. Our understanding of metabolic rhythms has been greatly enhanced by recent advances in high-throughput systems biology experimental techniques and data analysis. In an in vitro setting, metabolite rhythms can be measured by time-dependent sampling over an experimental period spanning one or more days at sufficent resolution to elucidate rhythms. We hypothesized that cellular metabolic effects over such a time course would be influenced by both oscillatory and circadian-independent cell metabolic effects. Here we use nuclear magnetic resonance (NMR) spectroscopy-based metabolic profiling of mammalian cell culture media of synchronized U2 OS cells containing an intact transcriptional clock. The experiment was con2016-01-01T00:00:00Z2016 Jul2026-07-09T10:19:02.153Z2025-06-01T12:39:47.247ZS-EPMC50411222747237510.3390/metabo6030023