{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Wang H"],"pubmed_abstract":["Nanoprobes for combined optical and magnetic resonance imaging have tremendous potential in early cancer diagnosis. Gold nanoparticles (AuNPs) co-doped with Gd<sub>2</sub>O<sub>3</sub> mesoporous silica nanocomposite (Au/Gd@MCM-41) can produce pronounced contrast enhancement for T1 weighted image in magnetic resonance imaging (MRI). Here, we show the remarkably high sensitivity of Au/Gd@MCM-41 to the human poorly differentiated nasopharyngeal carcinoma (NPC) cell line (CNE-2) using fluorescence lifetime imaging (FLIM). The upconversion luminescences from CNE-2 and the normal nasopharyngeal (NP) cells (NP69) after uptake of Au/Gd@MCM-41 show the characteristic of two-photon-induced-radiative recombination of the AuNPs. The presence of the Gd<sup>3+</sup> ion induces a much shorter luminescence lifetime in CNE-2 cells. The interaction between AuNPs and Gd<sup>3+</sup> ion clearly enhances the optical sensitivity of Au/Gd@MCM-41 to CNE-2. Furthermore, the difference in the autofluorescence between CNE-2 and NP69 cells can be efficiently demonstrated by the emission lifetimes of Au/Gd@MCM-41 through the Forster energy transfers from the endogenous fluorophores to AuNPs. The results suggest that Au/Gd@MCM-41 may impart high optical resolution for the FLIM imaging that differentiates normal and high-grade precancers."],"journal":["Scientific reports"],"pagination":["34367"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5046069"],"repository":["biostudies-literature"],"pubmed_title":["High sensitivity of gold nanoparticles co-doped with Gd<sub>2</sub>O<sub>3</sub> mesoporous silica nanocomposite to nasopharyngeal carcinoma cells."],"pmcid":["PMC5046069"],"pubmed_authors":["Shao Y","Tian X","Hu W","Wang H","Liu C","Zhang L","Zhang S","Li L"],"additional_accession":[]},"is_claimable":false,"name":"High sensitivity of gold nanoparticles co-doped with Gd<sub>2</sub>O<sub>3</sub> mesoporous silica nanocomposite to nasopharyngeal carcinoma cells.","description":"Nanoprobes for combined optical and magnetic resonance imaging have tremendous potential in early cancer diagnosis. Gold nanoparticles (AuNPs) co-doped with Gd<sub>2</sub>O<sub>3</sub> mesoporous silica nanocomposite (Au/Gd@MCM-41) can produce pronounced contrast enhancement for T1 weighted image in magnetic resonance imaging (MRI). Here, we show the remarkably high sensitivity of Au/Gd@MCM-41 to the human poorly differentiated nasopharyngeal carcinoma (NPC) cell line (CNE-2) using fluorescence lifetime imaging (FLIM). The upconversion luminescences from CNE-2 and the normal nasopharyngeal (NP) cells (NP69) after uptake of Au/Gd@MCM-41 show the characteristic of two-photon-induced-radiative recombination of the AuNPs. The presence of the Gd<sup>3+</sup> ion induces a much shorter luminescence lifetime in CNE-2 cells. The interaction between AuNPs and Gd<sup>3+</sup> ion clearly enhances the optical sensitivity of Au/Gd@MCM-41 to CNE-2. Furthermore, the difference in the autofluorescence between CNE-2 and NP69 cells can be efficiently demonstrated by the emission lifetimes of Au/Gd@MCM-41 through the Forster energy transfers from the endogenous fluorophores to AuNPs. The results suggest that Au/Gd@MCM-41 may impart high optical resolution for the FLIM imaging that differentiates normal and high-grade precancers.","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Oct","modification":"2025-04-18T18:37:39.09Z","creation":"2019-03-27T02:25:40Z"},"accession":"S-EPMC5046069","cross_references":{"pubmed":["27694966"],"doi":["10.1038/srep34367"]}}