{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sendra L"],"funding":["Ministerio de EconomÃa y Competitividad"],"pagination":["e0163898"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5047531"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(10)"],"pubmed_abstract":["Introduction
Expressing exogenous genes after naked DNA delivery into hepatocytes might achieve sustained and high expression of human proteins. Tail vein DNA injection is an efficient procedure for gene transfer in murine liver. Hydrodynamic procedures in large animals require organ targeting, and improve with liver vascular exclusion. In the present study, two closed liver hydrofection models employing the human alpha-1-antitrypsin (hAAT) gene are compared to reference standards in order to evaluate their potential clinical interest.Material and methods
A solution of naked DNA bearing the hAAT gene was retrogradely injected in 7 pig livers using two different closed perfusion procedures: an endovascular catheterization-mediated procedure (n = 3) with infrahepatic inferior"],"journal":["PloS one"],"pubmed_title":["Studying Closed Hydrodynamic Models of \"In Vivo\" DNA Perfusion in Pig Liver for Gene Therapy Translation to Humans."],"pmcid":["PMC5047531"],"funding_grant_id":["SAF2011-27002"],"pubmed_authors":["Diaz A","Sendra L","Garcia-Gimeno MA","Marti-Bonmati L","Montalva E","Sanz P","Perez-Enguix D","Noguera I","Alino SF","Lopez-Andujar R","Miguel A","Herrero MJ","Perez J"],"additional_accession":[]},"is_claimable":false,"name":"Studying Closed Hydrodynamic Models of \"In Vivo\" DNA Perfusion in Pig Liver for Gene Therapy Translation to Humans.","description":"Introduction
Expressing exogenous genes after naked DNA delivery into hepatocytes might achieve sustained and high expression of human proteins. Tail vein DNA injection is an efficient procedure for gene transfer in murine liver. Hydrodynamic procedures in large animals require organ targeting, and improve with liver vascular exclusion. In the present study, two closed liver hydrofection models employing the human alpha-1-antitrypsin (hAAT) gene are compared to reference standards in order to evaluate their potential clinical interest.Material and methods
A solution of naked DNA bearing the hAAT gene was retrogradely injected in 7 pig livers using two different closed perfusion procedures: an endovascular catheterization-mediated procedure (n = 3) with infrahepatic inferior","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2025-05-18T12:24:00.223Z","creation":"2025-05-18T12:24:00.223Z"},"accession":"S-EPMC5047531","cross_references":{"pubmed":["27695064"],"doi":["10.1371/journal.pone.0163898"]}}