biostudies-literatureErshova ASState contract in frame of Russian Federal Goal program “Development of pharmaceutical and medical industry of Russian Federation”544https://www.ebi.ac.uk/biostudies/studies/S-EPMC5054610biostudies-literatureUnknown16(1)<h4>Background</h4>E protein of tick-borne encephalitis virus (TBEV) and other flaviviruses is located on the surface of the viral particle. Domain III of this protein seems to be a promising component of subunit vaccines for prophylaxis of TBE and kits for diagnostics of TBEV.<h4>Methods</h4>Three variants of recombinant TBEV E protein domain III of European, Siberian and Far Eastern subtypes fused with dextran-binding domain of Leuconostoc citreum KM20 were expressed in E. coli and purified. The native structure of domain III was confirmed by ELISA antibody kit and sera of patients with tick-borne encephalitis. Immunogenic and protective properties of the preparation comprising these recombinant proteins immobilized on a dextran carrier with CpG oligonucleotides as an adjuvant were invesBMC infectious diseasesRecombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice.PMC5054610GK № 14.N08.11.0003Lyaschuk AMTkachuk APKaryagina ASGmyl LVGalushkina ZMKozlovskaya LILunin VGGra OAKholodilov ISGintsburg ALErshova ASSergienko OVGrunina TMKarganova GGSavina DMGudov VPBartov MSfalseRecombinant domains III of Tick-Borne Encephalitis Virus envelope protein in combination with dextran and CpGs induce immune response and partial protectiveness against TBE virus infection in mice.<h4>Background</h4>E protein of tick-borne encephalitis virus (TBEV) and other flaviviruses is located on the surface of the viral particle. Domain III of this protein seems to be a promising component of subunit vaccines for prophylaxis of TBE and kits for diagnostics of TBEV.<h4>Methods</h4>Three variants of recombinant TBEV E protein domain III of European, Siberian and Far Eastern subtypes fused with dextran-binding domain of Leuconostoc citreum KM20 were expressed in E. coli and purified. The native structure of domain III was confirmed by ELISA antibody kit and sera of patients with tick-borne encephalitis. Immunogenic and protective properties of the preparation comprising these recombinant proteins immobilized on a dextran carrier with CpG oligonucleotides as an adjuvant were inves2016-01-01T00:00:00Z2016 Oct2026-06-11T06:28:29.429Z2019-03-27T02:26:08ZS-EPMC50546102771731810.1186/s12879-016-1884-5