biostudies-literatureUnknown88(6)Ryndock ENanosonics LtdUltrasound probes used in endocavitary procedures have been shown to be contaminated with high-risk HPV after routine use and HPV is also known to be resistant to some high level disinfectants (HLDs). This study compared efficacy of two leading ultrasound probe HLD methods; liquid ortho-phthalaldehyde (Cidex® OPA) and an automated device using sonicated hydrogen peroxide (trophon® EPR) against HPV16 and HPV18 in a hard-surface carrier test. Native HPV16 and HPV18 virions were generated in organotypic epithelial raft cultures. Viral lysates were dried onto carriers with a 5% (v/v) protein soil. Efficacy tests were performed against the automated device at 35% and 31.5% H2 O2 and 0.55% OPA in quadruplicate with matched input, neutralization, and cytotoxicity controls. Hypochlorite was includJournal of medical virology1076-80https://www.ebi.ac.uk/biostudies/studies/S-EPMC5063110biostudies-literatureSusceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes.PMC5063110Robison RMeyers CRyndock EfalseSusceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes.Ultrasound probes used in endocavitary procedures have been shown to be contaminated with high-risk HPV after routine use and HPV is also known to be resistant to some high level disinfectants (HLDs). This study compared efficacy of two leading ultrasound probe HLD methods; liquid ortho-phthalaldehyde (Cidex® OPA) and an automated device using sonicated hydrogen peroxide (trophon® EPR) against HPV16 and HPV18 in a hard-surface carrier test. Native HPV16 and HPV18 virions were generated in organotypic epithelial raft cultures. Viral lysates were dried onto carriers with a 5% (v/v) protein soil. Efficacy tests were performed against the automated device at 35% and 31.5% H2 O2 and 0.55% OPA in quadruplicate with matched input, neutralization, and cytotoxicity controls. Hypochlorite was includ2016-01-01T00:00:00Z2016 Jun2025-04-04T09:21:06.461Z2019-03-27T02:26:35ZS-EPMC50631102651986610.1002/jmv.24421