biostudies-literatureKim HYNIEHS NIH HHS212-223https://www.ebi.ac.uk/biostudies/studies/S-EPMC5067221biostudies-literatureUnknown65Understanding the underlying temporal and mechanistic responses to neurotoxicant exposures during sensitive periods of neuronal development are critical for assessing the impact of these exposures on developmental processes. To investigate the importance of timing of neurotoxicant exposure for perturbation of epigenetic regulation, we exposed human neuronal progenitor cells (hNPCs) to chlorpyrifos (CP) and sodium arsenite (As; positive control) during proliferation and differentiation. CP or As treatment effects on hNPCs morphology, cell viability, and changes in protein expression levels of neural differentiation and cell stress markers, and histone H3 modifications were examined. Cell viability, proliferation/differentiation status, and epigenetic results suggest that hNPCs cultures respReproductive toxicology (Elmsford, N.Y.)Differential epigenetic effects of chlorpyrifos and arsenic in proliferating and differentiating human neural progenitor cells.PMC5067221P01 ES009601P30 ES007033Wegner SHWorkman TPark JJKim HYPacheco SEGriffith WFaustman EMVan Ness KPHong SfalseDifferential epigenetic effects of chlorpyrifos and arsenic in proliferating and differentiating human neural progenitor cells.Understanding the underlying temporal and mechanistic responses to neurotoxicant exposures during sensitive periods of neuronal development are critical for assessing the impact of these exposures on developmental processes. To investigate the importance of timing of neurotoxicant exposure for perturbation of epigenetic regulation, we exposed human neuronal progenitor cells (hNPCs) to chlorpyrifos (CP) and sodium arsenite (As; positive control) during proliferation and differentiation. CP or As treatment effects on hNPCs morphology, cell viability, and changes in protein expression levels of neural differentiation and cell stress markers, and histone H3 modifications were examined. Cell viability, proliferation/differentiation status, and epigenetic results suggest that hNPCs cultures resp2016-01-01T00:00:00Z2016 Oct2025-04-27T01:49:58.355Z2019-03-27T02:26:53ZS-EPMC50672212752328710.1016/j.reprotox.2016.08.005