biostudies-literatureBandara MMedical Research CouncilBiotechnology and Biological Sciences Research Council21474-21484https://www.ebi.ac.uk/biostudies/studies/S-EPMC5076819biostudies-literatureUnknown291(41)The accessory Sec system in Streptococcus gordonii DL1 is a specialized export system that transports a large serine-rich repeat protein, Hsa, to the bacterial surface. The system is composed of core proteins SecA2 and SecY2 and accessory Sec proteins Asp1-Asp5. Similar to canonical SecYEG, SecY2 forms a channel for translocation of the Hsa adhesin across the cytoplasmic membrane. Accessory Sec proteins Asp4 and Asp5 have been suggested to work alongside SecY2 to form the translocon, similar to the associated SecY, SecE, and SecG of the canonical system (SecYEG). To test this theory, S. gordonii secY2, asp4, and asp5 were co-expressed in Escherichia coli The resultant complex was subsequently purified, and its composition was confirmed by mass spectrometry to be SecY2-Asp4-Asp5. Like SecYEThe Journal of biological chemistryComposition and Activity of the Non-canonical Gram-positive SecY2 Complex.PMC50768191168597MC_U105178788BB/I008675/1Corey RACollinson ISkehel JMJenkinson HFBandara MBlocker AJMartin RfalseComposition and Activity of the Non-canonical Gram-positive SecY2 Complex.The accessory Sec system in Streptococcus gordonii DL1 is a specialized export system that transports a large serine-rich repeat protein, Hsa, to the bacterial surface. The system is composed of core proteins SecA2 and SecY2 and accessory Sec proteins Asp1-Asp5. Similar to canonical SecYEG, SecY2 forms a channel for translocation of the Hsa adhesin across the cytoplasmic membrane. Accessory Sec proteins Asp4 and Asp5 have been suggested to work alongside SecY2 to form the translocon, similar to the associated SecY, SecE, and SecG of the canonical system (SecYEG). To test this theory, S. gordonii secY2, asp4, and asp5 were co-expressed in Escherichia coli The resultant complex was subsequently purified, and its composition was confirmed by mass spectrometry to be SecY2-Asp4-Asp5. Like SecYE2016-01-01T00:00:00Z2016 Oct2026-04-14T17:05:35.563Z2019-03-27T02:27:23ZS-EPMC50768192755104610.1074/jbc.M116.729806