{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kuniyoshi Y"],"funding":["Core Research for Evolutional Science and Technology, Japan Science and Technology Agency","Japan Society for the Promotion of Science"],"pagination":["e0165473"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5082856"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(10)"],"pubmed_abstract":["Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding sequences (CDSs) but it has been difficult to process a number of hybridomas both with accuracy and rapidness. Here, we propose a new strategy for antibody sequence determination by mRNA-seq of hybridomas. We demonstrated that hybridomas highly expressed the Igh and Igl/Igk genes and that de novo transcriptome assembly using mRNA-seq data enabled identification of the CDS of both Igh and Igl/Igk accurately. Furthermore, we estimated that only 30,000 sequenced reads are required to identify immunoglobulin sequences from four different hybrido"],"journal":["PloS one"],"pubmed_title":["Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas."],"pmcid":["PMC5082856"],"funding_grant_id":["16K18479, 16H01577, 16H01550","25116010, 26290064","15K18457, 16H01219"],"pubmed_authors":["Iwasaki T","Kimura H","Fujita M","Maehara K","Kuniyoshi Y","Harada A","Ohkawa Y","Sato Y","Hayashi M","Semba Y"],"additional_accession":[]},"is_claimable":false,"name":"Identification of Immunoglobulin Gene Sequences from a Small Read Number of mRNA-Seq Using Hybridomas.","description":"Identification of immunoglobulin genes in hybridomas is essential for producing antibodies for research and clinical applications. A couple of methods such as RACE and degenerative PCR have been developed for determination of the Igh and Igl/Igk coding sequences (CDSs) but it has been difficult to process a number of hybridomas both with accuracy and rapidness. Here, we propose a new strategy for antibody sequence determination by mRNA-seq of hybridomas. We demonstrated that hybridomas highly expressed the Igh and Igl/Igk genes and that de novo transcriptome assembly using mRNA-seq data enabled identification of the CDS of both Igh and Igl/Igk accurately. Furthermore, we estimated that only 30,000 sequenced reads are required to identify immunoglobulin sequences from four different hybrido","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2026-04-29T16:49:36.989Z","creation":"2019-03-26T22:51:08Z"},"accession":"S-EPMC5082856","cross_references":{"pubmed":["27788226"],"doi":["10.1371/journal.pone.0165473"]}}