biostudies-literatureBelsey NADepartment for Business, Innovation and SkillsNIBIB NIH HHSEuropean Association of National Metrology InstitutesNational Research Foundation of KoreaEuropean CommissionNational Institute of Biomedical Imaging and BioengineeringDivision of Graduate EducationEngineering and Physical Sciences Research Council24070-24079https://www.ebi.ac.uk/biostudies/studies/S-EPMC5093768biostudies-literatureUnknown120(42)We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) inter-laboratory study on the measurement of the shell thickness and chemistry of nanoparticle coatings. Peptide-coated gold particles were supplied to laboratories in two forms: a colloidal suspension in pure water and; particles dried onto a silicon wafer. Participants prepared and analyzed these samples using either X-ray photoelectron spectroscopy (XPS) or low energy ion scattering (LEIS). Careful data analysis revealed some significant sources of discrepancy, particularly for XPS. Degradation during transportation, storage or sample preparation resulted in a variability in thickness of 53 %. The calculation method chosen by XPS participants contributed a variability of 67 %. However, variability The journal of physical chemistry. C, Nanomaterials and interfacesVersailles Project on Advanced Materials and Standards Interlaboratory Study on Measuring the Thickness and Chemistry of Nanoparticle Coatings Using XPS and LEIS.PMC50937681321276DGE-1256082EP/H006060/1P41 EB0020272014M3A7B602016314IND12HLT04EB-002027Galhardo CESturm JMNunney TSFearn SWang HAraujo JRShard AGWagstaffe MDietrich PMWang MWon Kim JSpampinato VBelsey NAPseiner JBock BBruner PCastner DGEngelhard MHLartundo-Rojas LLuftman HSCeccone GYang LMinelli CWang YCCounsell JDCant DJThomas AGWerner WVeith LSmith EFTreacy JPKalbe HfalseVersailles Project on Advanced Materials and Standards Interlaboratory Study on Measuring the Thickness and Chemistry of Nanoparticle Coatings Using XPS and LEIS.We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) inter-laboratory study on the measurement of the shell thickness and chemistry of nanoparticle coatings. Peptide-coated gold particles were supplied to laboratories in two forms: a colloidal suspension in pure water and; particles dried onto a silicon wafer. Participants prepared and analyzed these samples using either X-ray photoelectron spectroscopy (XPS) or low energy ion scattering (LEIS). Careful data analysis revealed some significant sources of discrepancy, particularly for XPS. Degradation during transportation, storage or sample preparation resulted in a variability in thickness of 53 %. The calculation method chosen by XPS participants contributed a variability of 67 %. However, variability 2016-01-01T00:00:00Z2016 Oct2025-04-03T22:39:33.83Z2019-03-27T02:28:07ZS-EPMC50937682781871910.1021/acs.jpcc.6b06713