{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bartels MF"],"funding":["Bundesministerium für Bildung und Forschung","Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen","Deutsche Forschungsgemeinschaft","Wellcome Trust","Senatsverwaltung für Wirtschaft, Technologie und Forschung des Landes Berlin"],"pagination":["e0166119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5094735"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(11)"],"pubmed_abstract":["Protein O-mannosylation is a post-translational modification essential for correct development of mammals. In humans, deficient O-mannosylation results in severe congenital muscular dystrophies often associated with impaired brain and eye development. Although various O-mannosylated proteins have been identified in the recent years, the distribution of O-mannosyl glycans in the mammalian brain and target proteins are still not well defined. In the present study, rabbit monoclonal antibodies directed against the O-mannosylated peptide YAT(α1-Man)AV were generated. Detailed characterization of clone RKU-1-3-5 revealed that this monoclonal antibody recognizes O-linked mannose also in different peptide and protein contexts. Using this tool, we observed that mono-O-mannosyl glycans occur ubiqui"],"journal":["PloS one"],"pubmed_title":["Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates."],"pmcid":["PMC5094735"],"funding_grant_id":["Sonderforschungsbereich 1036, project 11","WT099197MA","WT093378MA, WT099197MA and 108430/Z/15/Z","108430/Z/15/Z","WT093378MA"],"pubmed_authors":["Liu Y","Feizi T","Strahl S","Winterhalter PR","Mohrlen F","Hu H","Bartels MF","Westerlind U","Ruppert T","Lommel M","Yu J"],"additional_accession":[]},"is_claimable":false,"name":"Protein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates.","description":"Protein O-mannosylation is a post-translational modification essential for correct development of mammals. In humans, deficient O-mannosylation results in severe congenital muscular dystrophies often associated with impaired brain and eye development. Although various O-mannosylated proteins have been identified in the recent years, the distribution of O-mannosyl glycans in the mammalian brain and target proteins are still not well defined. In the present study, rabbit monoclonal antibodies directed against the O-mannosylated peptide YAT(α1-Man)AV were generated. Detailed characterization of clone RKU-1-3-5 revealed that this monoclonal antibody recognizes O-linked mannose also in different peptide and protein contexts. Using this tool, we observed that mono-O-mannosyl glycans occur ubiqui","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016","modification":"2026-05-02T02:25:53.91Z","creation":"2019-03-26T22:51:10Z"},"accession":"S-EPMC5094735","cross_references":{"pubmed":["27812179"],"doi":["10.1371/journal.pone.0166119"]}}