biostudies-literatureBartels MFBundesministerium für Bildung und ForschungMinisterium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-WestfalenDeutsche ForschungsgemeinschaftWellcome TrustSenatsverwaltung für Wirtschaft, Technologie und Forschung des Landes Berline0166119https://www.ebi.ac.uk/biostudies/studies/S-EPMC5094735biostudies-literatureUnknown11(11)Protein O-mannosylation is a post-translational modification essential for correct development of mammals. In humans, deficient O-mannosylation results in severe congenital muscular dystrophies often associated with impaired brain and eye development. Although various O-mannosylated proteins have been identified in the recent years, the distribution of O-mannosyl glycans in the mammalian brain and target proteins are still not well defined. In the present study, rabbit monoclonal antibodies directed against the O-mannosylated peptide YAT(α1-Man)AV were generated. Detailed characterization of clone RKU-1-3-5 revealed that this monoclonal antibody recognizes O-linked mannose also in different peptide and protein contexts. Using this tool, we observed that mono-O-mannosyl glycans occur ubiquiPloS oneProtein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates.PMC5094735Sonderforschungsbereich 1036, project 11WT099197MAWT093378MA, WT099197MA and 108430/Z/15/Z108430/Z/15/ZWT093378MALiu YFeizi TStrahl SWinterhalter PRMohrlen FHu HBartels MFWesterlind URuppert TLommel MYu JfalseProtein O-Mannosylation in the Murine Brain: Occurrence of Mono-O-Mannosyl Glycans and Identification of New Substrates.Protein O-mannosylation is a post-translational modification essential for correct development of mammals. In humans, deficient O-mannosylation results in severe congenital muscular dystrophies often associated with impaired brain and eye development. Although various O-mannosylated proteins have been identified in the recent years, the distribution of O-mannosyl glycans in the mammalian brain and target proteins are still not well defined. In the present study, rabbit monoclonal antibodies directed against the O-mannosylated peptide YAT(α1-Man)AV were generated. Detailed characterization of clone RKU-1-3-5 revealed that this monoclonal antibody recognizes O-linked mannose also in different peptide and protein contexts. Using this tool, we observed that mono-O-mannosyl glycans occur ubiqui2016-01-01T00:00:00Z20162026-05-02T02:25:53.91Z2019-03-26T22:51:10ZS-EPMC50947352781217910.1371/journal.pone.0166119