biostudies-literatureYan HNational Natural Science Foundation of China1786https://www.ebi.ac.uk/biostudies/studies/S-EPMC5099522biostudies-literatureUnknown7Increased evidences have demonstrated that gut microbiota targeted diet intervention can alleviate obesity and related metabolic disorders. The underlying mechanism of interactions among diet, microbiota, and host still remains unclear. <i>Enterobacter cloacae</i> B29, an endotoxin-producing strain dominated in the gut of a morbidly obese volunteer (weight 174.8 kg, BMI 58.8 kg m^-2) was isolated and transplanted to germfree mice (inoculated 10¹⁰ cells of B29 per day for 1 week). Using deep mRNA sequencing technology, we compared different gene expression profiles in the colon samples of the germfree mice treated with/without B29 and/or high fat diet (HFD) for 16 weeks and identified 279 differential expressed genes in total, including up-regulated genes <i>Apoa4</i> (Frontiers in microbiologyRegulated Inflammation and Lipid Metabolism in Colon mRNA Expressions of Obese Germfree Mice Responding to <i>Enterobacter cloacae</i> B29 Combined with the High Fat Diet.PMC509952231330005, 20875061Fei NZhao LZhang MWu GZhang CYan HfalseRegulated Inflammation and Lipid Metabolism in Colon mRNA Expressions of Obese Germfree Mice Responding to <i>Enterobacter cloacae</i> B29 Combined with the High Fat Diet.Increased evidences have demonstrated that gut microbiota targeted diet intervention can alleviate obesity and related metabolic disorders. The underlying mechanism of interactions among diet, microbiota, and host still remains unclear. <i>Enterobacter cloacae</i> B29, an endotoxin-producing strain dominated in the gut of a morbidly obese volunteer (weight 174.8 kg, BMI 58.8 kg m^-2) was isolated and transplanted to germfree mice (inoculated 10¹⁰ cells of B29 per day for 1 week). Using deep mRNA sequencing technology, we compared different gene expression profiles in the colon samples of the germfree mice treated with/without B29 and/or high fat diet (HFD) for 16 weeks and identified 279 differential expressed genes in total, including up-regulated genes <i>Apoa4</i> (2016-01-01T00:00:00Z20162026-04-07T15:46:31.478Z2019-03-27T02:28:29ZS-EPMC50995222787717210.3389/fmicb.2016.01786