{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["32(5)"],"submitter":["Park KS"],"pubmed_abstract":["<h4>Objective</h4>Fast detection of β-lactamase (<i>bla</i>) genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited <i>bla</i> gene types, these methods have significant limitations, such as their failure to detect almost all clinically available <i>bla</i> genes. We have evaluated a further refinement of our fast and accurate molecular method, developed to overcome these limitations, using clinical isolates.<h4>Methods</h4>We have recently developed the efficient large-scale <i>bla</i> detection method (<sub>large-scale</sub><i>bla</i>Finder) that can detect <i>bla</i> gene types including almost all clinically available 1,352 <i>bla</i> genes with perfect specificity and sensitivity. Using this method, w"],"journal":["Pakistan journal of medical sciences"],"pagination":["1309-1311"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5103155"],"repository":["biostudies-literature"],"pubmed_title":["Why cannot a β-lactamase gene be detected using an efficient molecular diagnostic method?"],"pmcid":["PMC5103155"],"pubmed_authors":["Park M","Lee SH","Karim AM","Park KS","Lee JH"],"additional_accession":[]},"is_claimable":false,"name":"Why cannot a β-lactamase gene be detected using an efficient molecular diagnostic method?","description":"<h4>Objective</h4>Fast detection of β-lactamase (<i>bla</i>) genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited <i>bla</i> gene types, these methods have significant limitations, such as their failure to detect almost all clinically available <i>bla</i> genes. We have evaluated a further refinement of our fast and accurate molecular method, developed to overcome these limitations, using clinical isolates.<h4>Methods</h4>We have recently developed the efficient large-scale <i>bla</i> detection method (<sub>large-scale</sub><i>bla</i>Finder) that can detect <i>bla</i> gene types including almost all clinically available 1,352 <i>bla</i> genes with perfect specificity and sensitivity. Using this method, w","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Sep-Oct","modification":"2025-05-29T20:20:37.668Z","creation":"2025-05-29T20:20:37.668Z"},"accession":"S-EPMC5103155","cross_references":{"pubmed":["27882043"],"doi":["10.12669/pjms.325.9837"]}}