biostudies-literatureUnknown32(5)Park KS<h4>Objective</h4>Fast detection of β-lactamase (<i>bla</i>) genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited <i>bla</i> gene types, these methods have significant limitations, such as their failure to detect almost all clinically available <i>bla</i> genes. We have evaluated a further refinement of our fast and accurate molecular method, developed to overcome these limitations, using clinical isolates.<h4>Methods</h4>We have recently developed the efficient large-scale <i>bla</i> detection method (_large-scale<i>bla</i>Finder) that can detect <i>bla</i> gene types including almost all clinically available 1,352 <i>bla</i> genes with perfect specificity and sensitivity. Using this method, wPakistan journal of medical sciences1309-1311https://www.ebi.ac.uk/biostudies/studies/S-EPMC5103155biostudies-literatureWhy cannot a β-lactamase gene be detected using an efficient molecular diagnostic method?PMC5103155Park MLee SHKarim AMPark KSLee JHfalseWhy cannot a β-lactamase gene be detected using an efficient molecular diagnostic method?<h4>Objective</h4>Fast detection of β-lactamase (<i>bla</i>) genes can minimize the spread of antibiotic resistance. Although several molecular diagnostic methods have been developed to detect limited <i>bla</i> gene types, these methods have significant limitations, such as their failure to detect almost all clinically available <i>bla</i> genes. We have evaluated a further refinement of our fast and accurate molecular method, developed to overcome these limitations, using clinical isolates.<h4>Methods</h4>We have recently developed the efficient large-scale <i>bla</i> detection method (_large-scale<i>bla</i>Finder) that can detect <i>bla</i> gene types including almost all clinically available 1,352 <i>bla</i> genes with perfect specificity and sensitivity. Using this method, w2016-01-01T00:00:00Z2016 Sep-Oct2025-05-29T20:20:37.668Z2025-05-29T20:20:37.668ZS-EPMC51031552788204310.12669/pjms.325.9837