biostudies-literatureWibmer CKFIC NIH HHSNIAID NIH HHS10220-10235https://www.ebi.ac.uk/biostudies/studies/S-EPMC5105658biostudies-literatureUnknown90(22)All HIV-1-infected individuals develop strain-specific neutralizing antibodies to their infecting virus, which in some cases mature into broadly neutralizing antibodies. Defining the epitopes of strain-specific antibodies that overlap conserved sites of vulnerability might provide mechanistic insights into how broadly neutralizing antibodies arise. We previously described an HIV-1 clade C-infected donor, CAP257, who developed broadly neutralizing plasma antibodies targeting an N276 glycan-dependent epitope in the CD4 binding site. The initial CD4 binding site response potently neutralized the heterologous tier 2 clade B viral strain RHPA, which was used to design resurfaced gp120 antigens for single-B-cell sorting. Here we report the isolation and structural characterization of CAP257-RH1,Journal of virologyStructure of an N276-Dependent HIV-1 Neutralizing Antibody Targeting a Rare V5 Glycan Hole Adjacent to the CD4 Binding Site.PMC5105658D43 TW000231U19 AI051794U01 AI116086Bailer RTSchmidt SDGorman JLouder MKAbdool Karim SSLabuschagne PMoore PLDruz AYork TMascola JRWilliamson CMorris LAnthony CSMkhize NNWibmer CKKwong PDfalseStructure of an N276-Dependent HIV-1 Neutralizing Antibody Targeting a Rare V5 Glycan Hole Adjacent to the CD4 Binding Site.All HIV-1-infected individuals develop strain-specific neutralizing antibodies to their infecting virus, which in some cases mature into broadly neutralizing antibodies. Defining the epitopes of strain-specific antibodies that overlap conserved sites of vulnerability might provide mechanistic insights into how broadly neutralizing antibodies arise. We previously described an HIV-1 clade C-infected donor, CAP257, who developed broadly neutralizing plasma antibodies targeting an N276 glycan-dependent epitope in the CD4 binding site. The initial CD4 binding site response potently neutralized the heterologous tier 2 clade B viral strain RHPA, which was used to design resurfaced gp120 antigens for single-B-cell sorting. Here we report the isolation and structural characterization of CAP257-RH1,2016-01-01T00:00:00Z2016 Nov2026-05-02T02:34:19.611Z2019-03-27T02:28:50ZS-EPMC51056582758198610.1128/JVI.01357-16