{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mohl BP"],"funding":["Wellcome Trust"],"pagination":["680-693"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC5115167"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["97(3)"],"pubmed_abstract":["Hepatitis C virus (HCV) infection has been shown to induce autophagy but the mechanisms underpinning this process remain to be elucidated. Induction of autophagy requires the class III phosphatidylinositol 3-kinase, Vps34, which produces phosphatidylinositol 3-phosphate (PI3P) within the endoplasmic reticulum (ER) membrane. This recruits proteins with PI3P binding domains such as the double-FYVE-containing protein 1 (DFCP1). DFCP1 generates cup-shaped protrusions from the ER membrane, termed omegasomes, which provide a platform for the production of autophagosomes. Here we present data demonstrating that both Vps34 and DFCP1 are required for HCV genome replication, in the context of both a subgenomic replicon and virus infection, but did not affect virus entry or initial translation. Using"],"journal":["The Journal of general virology"],"pubmed_title":["Early events in the generation of autophagosomes are required for the formation of membrane structures involved in hepatitis C virus genome replication."],"pmcid":["PMC5115167"],"funding_grant_id":["096670","099759"],"pubmed_authors":["Harris M","Mankouri J","Bartlett C","Mohl BP"],"additional_accession":[]},"is_claimable":false,"name":"Early events in the generation of autophagosomes are required for the formation of membrane structures involved in hepatitis C virus genome replication.","description":"Hepatitis C virus (HCV) infection has been shown to induce autophagy but the mechanisms underpinning this process remain to be elucidated. Induction of autophagy requires the class III phosphatidylinositol 3-kinase, Vps34, which produces phosphatidylinositol 3-phosphate (PI3P) within the endoplasmic reticulum (ER) membrane. This recruits proteins with PI3P binding domains such as the double-FYVE-containing protein 1 (DFCP1). DFCP1 generates cup-shaped protrusions from the ER membrane, termed omegasomes, which provide a platform for the production of autophagosomes. Here we present data demonstrating that both Vps34 and DFCP1 are required for HCV genome replication, in the context of both a subgenomic replicon and virus infection, but did not affect virus entry or initial translation. Using","dates":{"release":"2016-01-01T00:00:00Z","publication":"2016 Mar","modification":"2025-04-19T03:03:33.707Z","creation":"2019-03-26T23:38:21Z"},"accession":"S-EPMC5115167","cross_references":{"pubmed":["26727924"],"doi":["10.1099/jgv.0.000387"]}}